Research Of Cell Separation Chips Based On Microfluidics

With the development of bio-medical equipments and bio-medicaltechnology, studies on cells are increasingly receiving great attention.However, one problem closely related to the cell study is how to effectivelyseparate the specific cells from complex biological samples to reduce theinterference of other cells or materials with the results.The paper developed three different microfluidic cell separation chipsbased on different principles with MEMS technology. And it took mouseblood to study the separation of red blood cells and white blood cells. Thecontents include:1. Summarized the current domestic and international development ofvariety of cell separation chip technologies at the aspects ofbiological, physical and chemical methods, respectively. Comparedthe advantages and disadvantages of each separation techniques andproposed the research program of this paper.2. Based on the technology of MEMS and molding of plastic materials,studied the fabrication process of PDMS microfluidic chips withhigh aspect ration structure.3. Simulated the flow field and diffusion field at micro-scale with thesoftware CFD-ACE+. Analyzed the influence of different flow rateon mixing. Developed a micro-mixer based on the principle of Deanflow, which can speed up the mixing of blood and red blood cell lysisbuffer. 4. Developed a microfluidic chip based on cell size separationaccording to the principle of deterministic lateral displacement.Stained the mouse leukocytes with acridine orange to test theseparation effect of the chip.5. Simulated the distribution of flow rate in microfluidic chips withdifferent flow resistance ration of main channel to side channel.Analyzed the effects of flow resistance on cell separation. Anddeveloped a microfluidc cell separation chip based on the principleof Zweifach-Fung effect. Tested the separation effect of the chip withmouse blood.Although the paper just focused on the separation of mouse red bloodcells and white blood cells, these techniques also can be applied to theseparation of other cells. The results of this study on cell separation chiptechnologies laid a foundation for further studies.