Yin Fluid Side Effect On The Treatment Of Dry Eyes Mice Observation And Physiological Pathology

Purpose：Yangyin Shengjin Decoction on treatment of dry eye in mice wasobserved and the physiological and pathological effects.Material and method：1. Material：1.1Yangyin Shengjin decoction, Chinese medicine bureau by the AffiliatedHospital of Liaoning University of Traditional Chinese Medicine to provide rawmaterials.1.2SPF Kunming mice from China Medical University, Department of laboratoryanimal for animal，animal license key：SCXK（辽）2008-0005。1.3Sodium Hyaluronate Eye Drops,towering pharmacy stock, production batchnumber:1TH38781.4Rat pellet feed, purchased from the Beijing branch of the Australiancooperative feed Limited company, license key：：SCXK（京）2009-0012。2. Method：2.1According to Yangyin Shengjin decoction can be made of maximum drugconcentration and the mice maximum gastric perfusion volume, determination ofthe mice maximum dose.2.2Through the repeated injection of chemical drugs caused by insufficientsecretion of animal tear volume method to examine the role of drug treatments.2.2.1Establishment of a Murine model of dry eye.2.2.2Observation on effect of dry eye tear secretion in mice;2.2.3By fluorescent staining of cornea dry eye rats ’ observation on the effectof corneal epithelium;2.2.4Observation on histopathological changes and ocular conjunctiva changesof number of Goblet cells. 3.Results：3.1. Effects of Yangyin Shengjin. To make the maximum concentration is1.25g/ml, the largest volume in mice gavage of40ml/kg cases, mice via intragastricadministration, a days to maximum dose of50g (drug amount)/kg, is217timesthe amount of daily oral administration of adult.3.2. Yangyin Shengjin prescriptions can significantly increase the secretionof tears;3.3. Dry eye model has significant role in repair of corneal epithelium in mice;3.4. Histopathologic observation of ocular surface Epithelial Defects and promptsuppression of conjunctiva, angle.4.Conclusion：1. Yangyin Shengjin decoction can effectively increase in tear secretion, which,in two high dose group and the low dosage group and control group weresignificantly different (P <0.01). Tips of Yangyin Shengjin decoction canpromote the secretion of tear.2. Yangyin Shengjin prescriptions, high in both dose groups are significantlyreduced corneal epithelium of the fluorescent dye, compared with the blankcontrol group has significant differences (p﹤0.05,P﹤0.01). Tip yangyinShengjin prescription corneal nutritional status has improved, acceleratedrecovery of e-cadherin.3. Through the observation of ocular pathology, yangyin Shengjin prescriptionconjunctival organization does not fully back to normal, but to the point ofconfrontation of the conjunctiva, compared with the blank group significantdifference (p﹤0.05,P﹤0.01). Indicates yangyin Shengjin recipe can be partof angle of conjunctiva and corneal epithelial cells by inhibiting fell off.