| Objective: To investigate the effect of Yiqi Yangyin Qingre Recipe on the expression of GLP-2 and tight junction proteins ZO-1 and Occludin in type 2diabetes mellitus(T2DM)mice and to explore the effects of Yiqi Yangyin Qingre Recipe Inhibits chronic inflammatory responses and improves insulin resistance.Methods: Spontaneous type 2 diabetes KKAy mice with the similar pathogenesis of type 2 diabetes in humans were selected as animal models.Eighteen-week-old male mice with randomized glycemia(13.9 mmol/L)were divided into models according to the principle of randomization.Group,metformin group,Yiqiyangyinqingfang high-,middle-,and low-dose group,C57BL/6J mice were used as normal control group,10 mice in each group.The normal group and the model group were given 0.9% physiological saline.The Chinese medicine group was given high(2g/kg/d),medium(1g/kg/d),low(0.5g/kg/d)doses of Yiqi Yangyin Clearing Heat.Fangfu,metformin group to metformin tablets dissolved(200mg/kg/d)gavage.When the mice were 8 weeks old,10 weeks old,12 weeks old,14 weeks old,and 16 weeks old,the general condition of each group of mice was observed and body weight,fasting,and random blood glucose levels were measured;At the age of 24 weeks,the ultrastructure of intestinal mucosa and the morphological changes of Langerhans cells in each group were observed by transmission electron microscopy;GLP-2 and GLP-2 in mice at 16 weeks of age were measured by Western-blot.Connexin ZO-1,Occludin expression levels.Result:1.Compared with the normal group,the body weight,fasting,and random blood glucose levels of the KKAy mice in the model group continued to increase(P<0.01).Compared with the model group,the Chinese medicine group and metformin group can effectively reduce the body weight,fasting and random blood glucose(P<0.05).2.At the age of 16 th week,the intestinal mucosal cells of C57BL/6J mice in the normal group had no obvious changes,the inner and outer cell membranes were intact,the cell gaps were uniform,the size was normal,and the boundary was clear.On the other hand,the ultrastructure of the intestinal mucosa of the KKAy mice in the model group was destroyed,and the cell connection was opened.Langerhans cells(L)cells were not found.The metformin group was similar to the model group,but to a lesser degree.In the high-,middle-,and low-dose groups of Yiqi Yangyin Qingre Formula,the microvilli of the intestinal epithelium were densely organized,well-structured,and the cells were tightly connected with each other,and scattered L cells and Birbeck granules were found.3.At the 16 th week of age,compared with the normal group,the expression of GLP-2,ZO-1 and Occludin in the intestine of mice in the model group was significantly decreased(P<0.01).Compared with the model group,the expressions of GLP-2,ZO-1,and Occludin in the high,medium,and low dose groups of Chinese herbs were significantly increased(P<0.01),and were dose-dependent.The expression of GLP-2,ZO-1 and Occludin in the traditional Chinese medicine group was higher than that in the metformin group(P<0.05).Conclusion:1.Yiqi Yangyin Qingre Recipe can effectively reduce the body weight of type 2 diabetic KKAy mice,reduce their fasting and random blood glucose,and improve the general situation.2.The chronic inflammatory response and GLP-2 secretion disorders in the course of T2 DM may be related to the destruction of the ultrastructure of the intestinal mucosa,the opening of cell junctions,the increase of intestinal epithelial cell permeability,and the involvement of bacteria and toxins into the L-cells of blood damage.3.Yiqi Yangyin Qingre Recipe may improve intestinal GLP-2 and tight junction proteins ZO-1 and Occludin levels,improve the ultrastructure of the intestinal mucosa,maintain tight junctions between cells,protect intestinal L cells,and normal secretion GLP-2 achieves the purpose of inhibiting chronic inflammatory response,regulating sugar absorption homeostasis,improving insulin resistance,and lowering blood glucose. |
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