Study On The Protective Effects Of ZiNi Yangyin Shengjin Recipe On Radiation-Injured Mouse HPRT Gene

Objectives:In this study, mice were used as experimental subjects,30days survival rate ofmice by observing the detected radiation and the average survival days of mice ineach group hprt gene mutation rate and mutation spectrum. Explore ZiNi YangyinShengjin Recipe protective effect of the radiation damage mouse hprt gene. Rich intraditional Chinese medicine and radiation theory. To Chinese medicine prescriptionsprevent radiation damage detection experiments and theoretical guidance.Methods:Animal groups: healthy male mice were obtained200randomly divided into5group,(N=40). A. saline group B. radiation group1C. irradiated group2D. Chinesemedicine group1.E. Chinese medicine group2. Radiation:one-time given to Group B,Group D8Gy doses of X-ray. Repeatedly given to Group C, Group E total dose forX-ray8Gy,1Gy/d continuous exposure of8days. Administration: to group D. E.mice ZiNi Yangyin Shengjin Recipe on the intragastric.Times a day,0.5ml per eachDetection and observation: exposure period and continue administration until the endof the experiment.5days after radiation, groups of10mice were sacrificed.wereassayed hprt gene mutation rate and mutation spectrum. Observed in each group of30mice30days survival and average survival days.Results:1. Survival rate of mice and the average survival days. Each group of mice30dayssurvival rate: A. saline group100%，B. radiation group10.00%，C. radiationgroup213.3%，D. Chinese medicine group116.7%. E. Chinese medicine group226.7%.Each group of mice the average survival days: A. saline group30.0±0.0days，B. radiation group18.3±1.3days，C. radiation group213.1±4.2days，D. Chinese medicine group112.1±3.3days. E. Chinese medicine group216.4±1.7days，B. radiation group1and C. radiation group2mice30days of survival and mean survival days Compare d with the A. saline group were significantly lower, thedifference was significant (P <0.01)；Mice were given ZiNi Yangyin Shengjin Recipe,D. Chinese medicine group1survival and the average survival days Compare d with B.radiation group1significantly improved, the difference was significant (P <0.05); E.Chinese medicine group2survival and the average survival days Compare d with C.radiation group2significantly improved, the difference was significant (P <0.05);2. Each group of mice peripheral blood lymphocytes of hprt gene mutation rates：A.saline group0.35±0.02‰，B. radiation group10.80±0.07‰，C. radiation group20.98±0.02‰,D. Chinese medicine group10.72±0.02‰,E. Chinese medicine group20.85±0.01‰。B. radiation group1and C. radiation group2hprt gene mutation rateCompared with the A. saline group were significantly increased, the difference wassignificant (P <0.01)；Mice were given ZiNi Yangyin Shengjin Recipe, D. Chinesemedicine group1hprt gene mutation rate Compare d with B. radiation group1significantly lower, the difference was significant (P <0.05); E. Chinese medicinegroup2hprt gene mutation rate Compare d with C. radiation group2significantlylower, the difference was significant (P <0.05);3. Each group of mice peripheral blood lymphocytes the proportion of hprt genemutation spectrum of deletion mutations：A.saline group25％，B. radiation group175％C. radiation group287.5％D. Chinese medicine group150％，E. Chinesemedicine group262.5％. B. radiation group1and C.radiation group2the proportionof hprt gene mutation spectrum of deletion mutations Compare d with the A. salinegroup were significantly increased, the difference was significant (P <0.01)；Micewere given ZiNi Yangyin Shengjin Recipe,D. Chinese medicine group1the proportionof hprt gene mutation spectrum of deletion mutations Compare d with B. radiationgroup1significantly lower, the difference was significant (P <0.05); E. Chinesemedicine group2the proportion of hprt gene mutation spectrum of deletion mutationsCompared with C.radiation group2significantly lower, the difference was significant(P <0.05); Conclusion:1. ZiNi Yangyin Shengjin Recipe Can increase the survival of irradiated mice30daysand the average survival days.2. ZiNi Yangyin Shengjin Recipe Can reduce the hprt gene mutation rate of irradiatedmice.3. ZiNi Yangyin Shengjin Recipe Can Reduce the radiation mouse hprt gene mutationspectrum of the proportion deletion mutations.