Ultrasound-based Assessment In Early Atherosclerosis Rabbit Model

Objective: Pathology as the gold standard, to explore quality intima-mediathickness on evaluation of imtima-media thickness of the feasibility, accuracy andevaluate of Quality Intima-media Thickness(QIMT) and Quality Arterial Stiffness(QAS)changes in the stages of different atherosclerosis pathology. Methods: Forty2-month-oldhealthy male New Zealand white rabbits, one week after the feeding of the basis ofparticle feed adaptability, randomly selected10normal controls,30fed with high fat diet.Normal controls10animals, selected after line measurement of blood of blood lipids, theultrasound QIMT and QAS to check the right carotid artery and pathology;30high-fatdiet animals, high-fat diet, respectively,4,8, and12weeks. Three times, each time toselect10animals, respectively, the same check. According to the vessel wall arteryatherosclerosis hardening of pathological classification will be the case group is dividedinto pathological normal group, atherosclerosis Ⅰ,Ⅱ,Ⅲ(the ASⅠ, the ASⅡ, the ASⅢ)group, each group all measurement of Quality intima-mediaThickness(QIMT),vascular diameter and the Distensibility Coefficient(DC), ComplianceCoefficient(CC), stiffness α, stiffness β, Pulse Wave Velocity(PWV), Local systolicPressure(LOCsys), the establishment of the Fisher discriminant function to draw theprojection map, original method and cross-validated validation; draw the ROC curve.Results: With the pathological stage aggravating, QIMT, α, β of the AS group wereincreasing, PWV and LOCsys in the AS Ⅱ group than normal group was significantlyhigher, and the AS Ⅲ group than in AS Ⅱ group also increased. Vascular diameter andCC of the AS Ⅲ group were changed significantly than the normal group. DC changedsignificantly. Two non-standard Fisher discriminant function is:Equation1=2.211QIMT-1.295vascular diameter+0.856DC;Equation2=-0.775QIMT+1.662vascular diameter+0.294DC, discriminant validity of Equation1,2accounted for97.8%and2.0%. Back to the original of method, validationwas shown, function diagnosis of pathological normal group, AS Ⅰgroup, AS Ⅱ groupand group Ⅲ sensitivity100%,80%,100%and90%, total correct judgment rate of92.5%;cross-validated validated validation were90%,80%,100%and90%, total correctjudgment rate of90%. ROC show that the area the ROC curve, which were QIMT,vascular diameter and DC, CC, α, β, PWV, LOCsys, respectively was0.957,0.792and0.600,0.177,0.920,0.933,0.933,0.907. The corresponding boundary values were220.50,2.130and0.015,0.035,4.81,9.62,6.33and104.75. Change the sensitivity andspecificity for identification of vascular wall AS sensitivity and specificity, respectivelywere86.7％and100％,76.7％and80％and50％and70％,90％and90％,83.3％and90.0％,83.3％and90.0％,90.0％and90.0％,80.0and90.0％. Conclusion: The QIMT canbe accurate and reliable to intima-media thickness, and QIMT&QAS can beevaluated early atherosclerosis. Provided a reliable basis for clinical diagnosis andtreatment and Evaluation of arterial structure and flexibility of function is simple, rapid,noninvasive measurement results precise advantages. QIMT and QAS are able toevaluate the structure and flexibility of functional changes of different pathologicalstages of atherosclerosis, the establishment of the Fisher discriminant validity can moreaccurately determine atherosclerosis rating.