Population Structure Of Japanese Marsh Warbler(Megalurus Pryeri) By Microsatellite

Japanese Marsh Warbler(Megalurus pryeri)is one of the special birds only distributed in east Asia.The population number is very rare.Nine microsatellite loci were used to investigate the Japanese Marsh Warbler which distributed in Zhalong Nation Natural Reserve of Heilongjiang(breeding population),Shuangtai River Mouth Nation Natural Reserve of Liaoning(breeding population), Chongming Island Nation Natural Reserve of Shanghai(breeding population),Najishan National Natural Reserve of Jiangxi(wintering population and breeding population) genetic diversity.78samples were used to achieve this target. Five microsatellite loci of Megalurus pryeri sinensis were selected from Megalurus pryeri pryeri including Mslp1, Mslp2, Mslp3, Mslp4, Mslp5;four microsatellite loci of Megalurus pryeri sinensis were selected from Paradoxornis heudei including Parh15, Parh21, Parh25, Parh30.1. Genetic diversity of the Megalurus pryeri populationNine microsatellite loci in four breeding populations and one wintering population were all successful get purpose DNA clips.The study results through to the Japanese Marsh Warbler showed:average alleles number(NA) per loci was8.44. The Ho of these loci ranged form0.038(Parh15) to0.818(Mslp2)(average=0.375)and HE ranged from0.073(Parh15) to0.882(Mslp2)(average=0.532). PIC for the9microsatellite loci ranged between0.085(Parh15) to0.886(Mslp2)(averaged=0.517).From these data indicate that Japanese Marsh Warbler population showed a moderate polymorphism.Results after Bonferroni fixed of Hardy-Weinberg equilibrium tests showed that four microsatellite loci still deviating from Hardy-Weinberg equilibrium(p<0.0056) in five populations:Mslp3deviating from Hardy-Weinberg equilibrium in ZL population,STHK population,NJSB population,NJSW population; Mslp5deviating from Hardy-Weinberg equilibrium in STHK population;Parh25deviating from Hardy-Weinberg equilibrium in ZL population,NJSB population,NJSW population;Parh30deviating from Hardy-Weinberg equilibrium in ZL population,CMD population,NJSB population,NJSW population.The imbalance of loci maybe due to inbreeding.2. Clustering analysis and gene flowNei’s genetic distance were estimated and used to conduct NJ dendrograms.CMD population is alone,STHK population is also alone,ZL population and NJSB population were in one cluster after that meeting with NJSW population as the third cluster.The clusterting order conformed to the Japanese Marsh Warbler genetic distance between population.Gene flow (NM)between each population ranged from2.9675to50.7704(average=13.5),so the date indicating a large amount of average gene flow among Japanese Marsh Warbler population which distributed in China.3. Population genetic differentiationAMOVA showed the five Japanese Marsh Warbler population genetic variation among groups is(FCT=0.0073)not significant, among populations within groups genetic variation (FSC=0.0241)is also not significant,within populations genetic variation(FST=0.9686) is significant.At present, Japanese Marsh Warbler is still belongs to one population in China.The variation is mostly found among Japanese Marsh Warbler’s individuals.