Relationship Between Physical Condition And Correlative Factors Of Middle-aged And Elderly People With Levels Of Band 3 Protein Of Qingdao

Objective Band 3 protein of erythrocyte membrane could be used to evaluate health status and oxidative stress. The objective of the present study is to investigate the relationship of physical condition and correlative factors of middle-aged and elderly people with levels of band 3. To analyze the levels of band 3 under different physio-pathologic status.Methods Random sampling method for selecting subjects. Physical examination data and blood samples of 218 middle-aged and elderly people (80 males and 138 females) were collected. SDS-polyacrylamide gel electrophoresis was used to analyze band 3 protein in erythrocyte membrane. The difference of band 3 protein in different groups was analyzed by T Test and One-Way ANOVA. Correlation and regression of band 3 protein with physiological status was analyzed by Pearson correlation and linear regression.Results In this study, the band 3 protein level of workers with medium activity was 24.09%, which was significantly higher than that of light activity workers (23.42%) (p< 0.05). The subjects with hypertension were proved to have a significantly lower level of band 3 protein (23.33%) than normal individuals (24.20%) (p<0.05). Compared with people who got hypertriglyceridemia or hypercholesterolemia(22.91%,23.19%), the levels of band 3 protein was significantly decrease than normal(23.98%,23.90%)(p< 0.05). There was a significantly positive correlation (r=0.149) between the level of band 3 protein and physical activity (p<0.05), and a negative correlation (r=-0.156) between the level of band 3 protein and systolic blood pressure (p<0.05). Levels of band 3 protein correlated negatively with the plasma levels of triglyceride (r=-0.166)and cholesterol (r=-0.176)(p<0.05). The correlation of band 3 protein with gender, age, BMI and diastolic blood pressure (p>0.05) was not significant. There were uncorrelated relationship between band 3 protein and the plasma levels of glucose, high-density lipoprotein, protein, liver function test, renal function test (p>0.05).Conclusion The level of band 3 protein was positively correlated with physical activity and negatively correlated with systolic blood pressure. Along with increase in levels of triglyceride and cholesterol, levels of band 3 protein was decrease. Levels of band 3 was different in many different physio-pathologic status.