Pcr-ce Chip Portable Analyzer Control System Development

Micro-fluidic chips are gaining significant popularity as they provide attractive solutions to automate and miniaturize the handling of fluids, reagents and other fluids used in DNA sample preparation, synthesis and screening. The PCR-CE micro-fluidic chip platform works step by step: At first, the biology solution is sampled by electroosmosis flow driven under a certain electric field between two platinum electrodes, then the sample flows through three different temperature zones namely 40-60°C for annealing, 70-80°C for extension and 90-95°C for denaturation at a high speed in a circle to realize the PCR process. At last, the sample is separated and detected by capillary electrophoresis.However, many micro-fluidic chips have to be used with the help of other expensive and big devices like photomicroscopes. The high cost on devices limits the application of micro-fluidic chips greatly. Thus, one fruitful way is to minimize the chips' device platform which can process data, run programmers, read the sensors and control TE and has pocket human-machine interface.This paper developed integrated polymerase chain reaction-capillary electrophoresis (PCR-CE) micro-fluidic chip device control system that can perform a series of analytical steps including PCR process, electroosmosis drive and CE separation. The miniaturized device control system was designed based on the embedded, 32 bites RISC microcontroller ARM (LPC2138). Because the ability to precisely control temperature is a critical component of PCR micro-fluidic devices, the fuzzy immune PID control arithmetic used in the device ensured the temperature control precise and robust. The device also had matrix keyboard, small LCD displayer, serial communication unit, and capillary electrophoresis (CE) separation unit. The software of the system included two parts: one was designed based on μc/os- II real time operation system, which scheduled the programs in the ARM flash; the other was developed on Visual C++ platform in PC, which could realize data setting, data analyst and communicate with the subsystem by serial...