Study On Fabrication And Fluidic Controlling Performance Of Lab-on-a-chip

In the thesis, the conception, advantage and limitation of the micro fluidic controllinganalyticat chip were introduced, and internal and overseas research works weresummarized. The main works and achievements are presented as follows.(1) The fabrication of the glass micro fluidic controlling analytical chips wasintroduced in detail, which included the selection of materials, the design of themicrostructure, the optical lithography, the wet chemical etching and the final bonding.Several major factors which affect the etching were compared and analyzed. Theconditions of the etching were optimized. Two bonding technologies for low temperatureor room temperature were introduced into the experiments. Primary technologies forfabricating the glass micro fluidic controlling analytical chips were achieved, which can beapplied in the general laboratories.(2) The electrokinetic driving theory of the micro fluid in the micro fluidic controllinganalytical chip was introduced. Based on the analysis of the theories on the Electric DoubleLayer (EDL) and Electroosmosis floyd, the two dimensional electrokinetic drivingmathematic model of micro fluid was established, and the stable analytic solutions of thePoisson-Boltzmann equation and the Navier-Stokes equation were deduced. Theelectroosmotic potential field, the near-wall potential field, the electro-osmotic flow rate,and the shear stress on the surface of the wall were simulated by Matlab software. Therelations of the above investigated objects with ionic energy parameterαand the variableβwere analyzed respectively, which demonstrated the validity of the electrokinetic drivingtheory.(3) The self-made glass micro fluidic controlling analytical chips were used toinvestigate the relationship between the micro fluid flow rate and the external voltage inthe micro channel with different equivalent width. Based on the comparative analysis ofthe flow fields in the micro channel of T-type and crisscross-type, the relations of thedistribution of the flow field to the external voltage, the superimposed electric-field, thedistance from the electrode, and the status of the electrode with applied voltage weresummarized respectively.