The Codonopsis Water Extract Of The Exercise Mice Quadriceps Energy Metabolism Indicators

1. ObjectiveBy observing the influence from codonopsis Lanceolata extract on training SDH and MDH (aerobic oxidation of the marker enzyme for energy systems),LDH (anaerobic oxidation of marker enzyme) and Ca2+-Mg2+-ATPase activity and muscle glycogen, this paper is to explore the impact of codonopsis Lanceolata extract which could improve the body's energy metabolism and the exercise capacity, and is to provide scientific basis for training with better guidance and for enhancing athletic ability, also is to offer theoretical basis for the development of Chinese herbal.2. MethodsKunming mice, male, weighting from 18g to 22g, are offered by Shandong Lukang Pharmaceutical Animal Experiment Center. The animal room temperature was 23-28℃, humidity was 40% -65%, lighting time was changing with time, and ventilation was good. Fed by mixed forage and cold water randomly and controlled strictly, the mice were divided into cages and 10 in each cage. After one-week adaptation feeding in the animal room, the mice were randomly divided into 4 major groups, including control group (A) medication group (B) training group (C) training+medication group (D), each group was 30, the total was 120. Based on the death state, each group was further divided into three subgroups:the quiet, the exhaustive status and recovery after exhaustive exercise 12h, and at the same time did decollation to get the quadriceps femora to assay muscle glycogen, lactic dehydrogenises(LDH), succinct dehydrogenises(SDH), malice dehydrogenises(MDH), and Ca2+-Mg2+-ATPase activity. All these data used SPSS 17.0 statistical software to analyze.3. Results3.1 Exhaustive time, the exhaustive time of training+medication group was significantly longer than the control group (P<0.01), and significantly longer than the medication group; the training group and medication group were significantly longer than the control group (P<0.05).3.2 resting state, compared with the control group, muscle glycogen content of training+ medication group was significantly higher (P<0.05); LDH activity of training+medication group was significantly higher, but had no significant differences with other groups; MDH activity of medication group was significantly higher (P<0.05), and training+medication group was significantly higher (P<0.01); SDH activity of each group was no significant differences; Ca2+-Mg2+-ATPase activity, there was no significant differences in each group.3.3 Exhaustive status, compared with the control group, muscle glycogen content of training group and training+medication group were significantly lower than the control group (P<0.05); LDH activity of training+medication group was significantly higher (P<0.05); MDH activity of training+medication group was significantly higher (P<0.01); SDH activity, medication group, training group and training+medication group were significantly higher (P<0.05); Ca2+-Mg2+-ATPase activity, medication group was significantly higher (P<0.05), both training group and training+medication group were significantly higher (P<0.01).3.4 Exhaustive recovery 12h, compared with the control group, muscle glycogen content of medication group was significantly higher (P<0.05), training+medication group was significantly higher (P<0.01); LDH activity of training+medication group was significantly higher (P<0.05); MDH activity of training group was significantly higher (P<0.05), also medication group and training+medication group were significantly higher (P<0.05); SDH activity of medication group and training+medication group were significantly higher (P<0.05); Ca2+-Mg2+-ATPase activity, medication group was significantly higher (P<0.05), training group and training+medication group were significantly higher (P<0.01), training group was significantly higher than the medication group (P<0.01), training+medication group was significantly higher than the medication group (P<0.05).4 Conclusions4.1 The exercise training could improve the muscle glycogen content of quadriceps femora, promote muscle glycogen synthesis during the recovery period, increase the activity of SDH and MDH in exhaustive state, improve aerobic oxidative capacity, speed up the repair of body damage, accelerate the elimination of inflammation, and improve athletic ability.4.2 Fed by extract of codonopsis Lanceolata could improve muscle glycogen content of quadriceps femora, promote muscle glycogen synthesis during the recovery period, increase the enzymes activity of MDH, SDH and Ca2+-Mg2+-ATPase activity under the status of exhaustion and recovery 12h, promote the recovery of Ca2+-Mg2+-ATPase, suggest that extract of codonopsis Lanceolata could enhance the body's aerobic capacity and improve the exercise capacity.4.3 Taking the extract of codonopsis Lanceolata and swimming to do combination, this way could increase the muscle glycogen reserve of quadriceps femora, improve the MDH and SDH activity in the resting state.