| Objective: By exogenous supplementing L-carnitine (L-carnitine) and combined with thelong-term swimming training, to observe the changes of the SD rat blood a number ofbiochemical indices and skeletal muscle mitochondrial enzyme activity after exercise, effectsof exercise training and supplementary cause and mechanism of L-carnitine on the impact ofthe body’s blood biochemical parameters and lipid metabolism, in order to provide a validexperimental basis and reference for research to improve the athletic ability of the body andimprove the body’s fat metabolism.Method: Healthy male peers purebred SD rats32, all rats in the adaptability of feeding after aweek, according to the pilot scheme were randomly divided into sedentary control group(group A), a simple exercise group (group E), a simple supplement carnitine group (group L),exercise combined with L-carnitine (group LE), sub-cage feeding, each group is8. Quietcontrol group and simply added L-carnitine does not make any movement, the simple exercisegroup and exercise combined with L-carnitine group swim training, the training time for sixweeks. Group L and training combined with the supplement L-carnitine group training threetimes a week, and one hour before each swim training supplement L-carnitine, group A andgroup E as well normal eating the same amount of rat food, After six weeks of experiments,the rats were decapitated to remove the blood and skeletal muscle samples, respectively, usinga UV spectrophotometer, ELISA, and kits for determination of the rats in each groupcorresponding blood biochemical indices and muscle tissue mitochondrial H~+K~+-ATPaseenzymes, CPT-I enzyme activity will be measured and analyzed.Results:(1) After6weeks of experiments, compared to the sedentary control group, group E,and exercise combined with L-carnitine weight of rats, the differences were statisticallysignificant (P <0.05); exercise combined with L-carnitine and group L, body weightdifference was statistically significant (P <0.05), compared to group E, exercise combinedwith L-carnitine group, rats body weight differences are not statistically significant (P>0.05).(2) Compared with the quiet control group, simple exercise group in rat skeletal musclemitochondrial H~+K~+-ATP enzyme of CPT-I enzyme activity differences not statisticallysignificant (P>0.05); simply supplement L-carnitine in rats H~+K~+-ATP enzyme of CPT-Ienzyme activity increased (P>0.05), but the difference was not statistically significant;activity of H~+K~+-ATPasethe and CPT-I enzyme in group LE increasing has statisticallysignificant (P <0.05). Compared with t group E, exercise combined with L-carnitine group ofH~+K~+-ATPase enzyme, the CPT-I activity was significantly increased, with statisticalsignificance (P <0.01).(3) Compared with the quiet control group, group E and LE serumcarnitine content differences were statistically significant (P <0.05), compared to LE rats,serum carnitine content reduction in group E was statistically significant, serum carnitinelevels in group LE increased statistically significant (P <0.05).Conclusions:(1) Swimming combined with L-carnitine to a certain degree of body weight ofrats decreased body serum carnitine content and free fatty acid content increased significantly,which is conducive to fat metabolism for energy capacity increase,may be one of the reasonsfor the reduced body weight of rats.(2) Compared to the quiet state, rats in group E and swimming combined with supplementaryL-Carnitine, serum free fatty acids increase more significantly, suggesting that swimmingstate muscle fatty acid uptake and use may significantly increase. (3) In the quiet and simple supplementing L-carnitine under the conditions of the differencesof serum carnitine content is not significant, andswimming combined with L-carnitine serumcarnitine decreased, suggesting that carnitine’s uptake and utilization increases. Exercise andcombined with L-carnitine increased skeletal muscle carnitine content, may cause an increasein speed of acyl-CoA into the mitochondria, thereby increasing fatty acid oxidation rate.(4) Compared to quiet rats, L-carnitine supplementation does not produce a significant impacton the rats of CPT-I enzyme activity, but exercise combined with L-carnitine, CPT-I enzymeactivity increased and is the most obvious, indicating that skeletal muscle cells duringexercise may speed up the rate of fatty acid oxidation.(5) Compared to quiet state, L-carnitine supplementation, swiming combined with H~+K~+-ATPase activity was significantly increased after supplementary L-carnitine in skeletalmuscle mitochondria, suggesting that L-carnitine supplement may be the body of H~+K~+-ATPase activity increased the effects of promoting skeletal muscle mitochondrial energymetabolism. |
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