| Objective: By selecting the8-week-old male KM mice for the study materials, the designof different intensity treadmill training and sea cucumber small peptides complementarycombination of solutions, testing training after the end of the mouse skeletal muscle pyruvatekinase (PK), Apple acid dehydrogenase (MDH) and succinate dehydrogenase (SDH)) activity,recording the exhaustive swimming time of mice in each group training is completed, thechange by exploring the metabolic enzymes of the energy supply system in the state of thedifferent forms of exercise and nutritional supplements characteristics and laws to reveal theimpact of long-term treadmill training on the energy metabolism enzymes in mouse skeletalmuscle to provide a reference for the development of anaerobic and aerobic capacity. Finally,we discuss the impact of sea cucumber of small peptides on the body’s resistance to fatigue, toprovide a reference for the development of sports nutrition.Methods:30male KM mice were randomly divided into control group (C), the aerobicexercise group (T1), anaerobic exercise group (T2), aerobic exercise, nutritional supplementgroup (T3), anaerobic exercise, nutritional supplementation group (T4),(n=6). Treadmillwith electric animals as training equipment, conventional T1, T2, T3, T4group fed for threedays, and then one week of adaptation training, speed for8m/min,10min/d, to adapt thetraining is completed,T1,T3mice for a period of six weeks of76%VO2max aerobic treadmilltraining, T2, T4, mice of95%VO2max, anaerobic treadmill training, the training intensity forthe ultimate strength training six days a week, incline is00. Mice in each group after training(6w) from the eye vein blood0.5~1.5ml blood centrifuge2500rpm for10minutes, thesupernatant was placed in4°C refrigerator to save for the determination of serum SOD inmice MDA. Were sacrificed after the completion of the venous blood, and quickly take theright leg quadriceps cleaning, drying to remove blood, filter paper, weighed, placed in a smallbeaker. Grinder in an ice bath under the conditions of full pulverisation, organized into thehomogenate.10%of the prepared tissue homogenates low temperature centrifuge2500rpmspeed centrifugation for10min, the supernatant was placed on the sample tube in-70°C coldstandby, the supernatants were used for the determination of various indicators. Using enzymeimmunoassay measuring mouse quadriceps, MDH, SDH, the PK activity. Each set of data,statistical processing, comparison and analysis. Results:(1) after6weeks of treadmill training and nutritional supplement, anaerobic training micequadriceps PK activity has increased, compared with the control group, T2, significantdifference (P <0.05) T4in group differences is very significant (P <0.01), T4and T2groupthe difference was significant (P <0.05); T3and T1compared to no significant change.(2) after6weeks of treadmill training and nutritional supplements, aerobic exercise groupmice quadriceps, MDH, SDH activity has increased compared with the control group, the T3group has a very significant difference (P <0.01), T1, group, significant changes (P <0.05),T3and T1group compared changes significantly (P <0.05); T4in comparison with the T2phase, no significant change.(3) after6weeks of treadmill training and nutritional supplements, aerobic exercise groupmice swimming exhaustive compared with control mice changed significantly (P <0.01);anaerobic training mice exhaustive swimming time with the control group compared to anincrease in T4in change significantly (P <0.05), T2, no significant changes, not statisticallysignificant; T3group with T1group compared to find significant changes (P <0.05), T4groupT2phase, there was significant difference (P <0.05).(4) after6weeks of treadmill training and nutritional supplements, found that serum SOD,MDA, a different degree of change. Of T1, T2, T4, the serum SOD levels change verysignificantly (P <0.01), significant changes in the T2group (P <0.05). Of T1, T2, T4, theserum MDA content was significantly (P <0.01), no significant change in T2group. The sameform of exercise between groups, significant differences (P <0.05).Conclusions:(1) long-term anaerobic exercise training can make the mouse skeletal musclePK activity increased, indicating that long-term anaerobic training by regulating the activityof metabolic enzymes to improve the glycolytic capacity.(2) long-term aerobic training can effectively improve the mouse skeletal muscle tissue SDH,MDH activity, indicating that long-term aerobic training can improve the body’s aerobicenergy capacity by increasing the citric acid cycle enzyme activities.(3) long-term aerobic training, aerobic metabolic activity was increased significantly; andoften a corresponding increase in enzyme activity of the anaerobic training anaerobicmetabolism.(4) added the sea cucumber small peptides can enhance the activity of SDH, MDH, PK, thismay be it is absorbed and converted into raw materials of the synthesis of enzymes of energymetabolism required for anti-fatigue effects metabolism ability, thereby regulating the MDH,SDH, PK activity is related to the specific mechanism is not clearly require further study.(5) added the sea cucumber small peptides can improve the mice resistance to fatigue, which may increase in the ability of anti-oxidation of small peptides of sea cucumber or related tothe improvement of energy metabolism enzymes in mice SDH, MDH, PK activity. |
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