| Objective: To study the influence of motor function of early intervention to reduce cerebral palsy neonatal rats caused by brain damage.Method: 1. Establishment of model and grouping: 48 Wistar pregnant rats were consecutively injected with LPS (450μg/kg) for two days on gestation 18 days. Control group pregnant rats were injected same dose of saline . The rats whose gestation age was less than 22 days were preterm rats and were removed in both groups. Selected randomly control group(A) neonatal rat(n=60), LPS group(n=120), divided LPS group into intervention group B1(n=60), nonintervention group B2(n=60). Three groups rats added nutrition regulary,breeded constant temperature and humidity, neonatal rats were feeded with breast milk. 2. Uterus and placenta and brain tissue pathology testing: After delivering rat, a part of female rats were executed. The uterus and placenta were taken out. The HE staining sections were examined under microscope for numbers of neutrophilic granulocyte in the uterus and placenta. After 24 hours about neonatal rats were born , neonatal rats of control group and the LPS group were excuted and we got the brain on the ice box. HE staining, observed the white matter injury. 3. The 2 days rats of intervention group were early touch, after one week were given enriched environment and after two weeks were given generally load swimming training up to six weeks. 4. Identification of cerebral palsy rats for 25-day-old :the rats in each group were respectively carried out neurobehavior detection and improved BBB motor function assesments,and statistical analysis. 5. 42-day-old control group rats , cerebral palsy rat of B1 group and B2 group,carry out suspension test and improved BBB motor function assessment,and statistical analysis.Results: 1. There were no dead or pre-parturition's pregnant rats in control group, the pregnant rats delivered all 108 live mature rats . 12 pregnant rats were dead after injected LPS. There were 10 pregnant rats of pre-parturition in LPS group. the rest of 26 pregnant rats delivered 138 live mature rats and 84 dead rats in LPS group. 2. Vascular congestion and edema and there were a large amount of neutrophilic granulocyte in the uterus and placenta in LPS-treated group. 1-day-old rat brain tissue pathology test showed:white matter of control group integrity,orderly distribution, nuclear desalination, clearly nucleolus. In LPS group, white matter, corpus callosum, internal capsule parts organizations osteoporosis, Glial cells gathered, Capsule of oligodendrocyte reduction,in white matter can also be found vasodilator,White matter capillary bleeding. 3. 25-day-old of neonatal rats neurological behavior, B1 group identified seven CP rats, B2 group identified thirteen CP rats. 4. 25-day-old of neonatal rats union applicates suspension test and improved BBB motor function assessment, B1 group identified seven CP rats, B2 group identified thirteen CP rats. 5. Comparison of the test results of the 25-day-old and 42 days of cerebral palsy rat in B1 group: suspension test, slopes test, open-field experiments, resist captured reaction p<0.01; improved BBB motor function score p<0.01; Comparison of the results showed no significant differences in cerebral palsy rats of B2 group and control group rats of 25-day-old and 42-day-old.Conclusion: 1. Union applicates suspension test and improved BBB motor function assessment is consistent with cerebral palsy rats identification and neurobehavioraldetection.2. Union applicates suspension test and improved BBB motor function assessment to improve motor function of cerebral palsy rats caused by intrauterine infection is superior to the neurobehavioral detection.3. Early intervention can reduce cerebral palsy neonatal rats caused by intrauterine infection, and can significantly improve the motor function of cerebral palsy neonatal rats. |
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