To Constructe And Assesst The Hypoxic Ischemic Sequential Intrauterine Infection Cerebral Palsy Rats Model

Objective To construct a novel type of cerebral palsy model in rats andanalyze the weight, eye opening, behavioral and neurpathological changeson postnatal28th day in order to explain the superiority of our model.Methods Randomly select10rats from15SD pregnant rats and their lateroffspring as the experimental group1,3from others as the experimentalgroup2and the other two as the control group.The pregnant rats wereinjected LPS by i.p. in the experimental group and saline solution in thecontrol group every two days from E16until delivery.4hours afterinjection, the experimental group was exposed to8%O2/N2mixture for2.5hours. On postnatal7th day, bilateral common carotid arteries were ligatedin the experimental group1. On postnatal14th and28th day, three groupsof pups were tested by weight, crawling ladder, MNSS, open fieldobservation,eye opening, grip test, rotarod test and pathological section.Results The experimental group pups showed slow grewth anddevelopment,the left eyes open day of are16.9±2.0、15.2±2.0and13.0±2.0in the three group and the rights are on23.1±2.6、15.3±2.1and13.7±1.7.The experimental group are with the score of2.7±0.1、1.5±0.4and1.5±0.4in grip test and the score of MNSS are4.9±2.5、4.1± 1.0and1.2±1.0.In open field observation,we find the experimental group1, the experimental group2and the control group have different indistance.(P<0.01). Neurons arrange in disorder, the white matter is thinnerand inflammatory cells infiltrated in the experimental group pups. Theexperimental group2is not as serious as the group1.Conclusion In late pregnancy intraperitoneal injection LPS and hypoxiawith postnatal bilateral common carotid artery ligation can build a newstable rat cerebral palsy model.