Early Intervention And Rehabilitation Training On Brain Injury In Mouse Tissues Mag Expression And The Influence Of Cerebral White Matter Myelinated Nerve Fiber Diameter

Objective: To observe the effect of early intervention for the MAG expression and thediameter of myelinated nerve fibersof brain injury neonatal rat caused by intrauterine infection.Methods:1. The establishment of animal models: select40Wistar female rats with10male rats. The female rats were randomly divided into two group: LPS-treated group (n=32)and saline group(n=8). Room temperature (21℃+1℃) with the conventional breeding, freefood, light and shade cycle evenly (12h/12h), adapt to the environment2weeks, we putfemale and male in one cage (proportion2:1)at17:00, at8:00the next morning, checkvaginal smear, minnesota sperm for pregnancy0day, pregnant mice was kept separately.At the17th and18th, pregnant rats of LPS-treated group were consecutively injected withLPS (420ug/kg), pregnant rats of saline group were consecutively injected with same dosessaline. To observe and record the delivery time of pregnant mouse and the condition ofnewborn pups. Randomly select newborn pups of LPS-treated group and newborn pups ofsaline group. Newborn pups of LPS-treated groupwere randomly divided into two group:intervention group (T,n=60), non-intervention group (NT,n=60). Mother was feed and fullycompensatory nutrition in constant temperature and humidity. Newborn pups were feed bybreastfeeding.2. Early intervention: newborn pups of T group were gave early interventionfrom the second day, including early touching, rich environmental stimulation andrehabilitation training. Newborn pups of NT group and NS group were fed by routine way.3.Praxiology and pathobiology detection: Detection the movement function changes of rats ineach group at the14days,21days,28days. HE stain were employed to observe thepathomorphology changes of placenta of pregnant rats and brain of offspring’s.4.Immunohistochemical detection: The immunohistochemical stain was used to detect the IODof MAG in brain on the1d,7d,14d,21d,28d of each group of pups.5.Stereology detection:using serology method to detect myelinated nerve fibers change of the total length on the14d,21d,28d of each group of pups..6. Statistical analysis of MAG expression and theirmyelinated nerve fiber diameter with or without correlation.Results:1. The23LPS group pregnant rats delivered138full-term pups and15dead,after injecting intraperi,5were dead and4ahead of delivery,The8normal saline pregnant ratsdelivered83full-term pups and none was died or pre-parturition.2. HE staining results: inLPS group of pregnant rats placental pathological examination we can see visible massiveneutrophil infiltration, vascular congestion and edema apparent inflammation; control group of rats placenta were normal; in LPS group brain tissue pathological examinationshowed obvious inflammatory changes, such as loose structure of the white matter,capillary dilatation and congestion or bleeding, glial cell aggregation, intraventricularhemorrhage and other obvious inflammatory changes; newborn brain tissue of the controlgroup have no significant pathological changes.3. Praxiology test results:①suspensiontest results: compared with the control group, the activities of the intervention group andthe non-intervention group was significantly reduced in balance and coordination ability,muscle tension, muscle, unresponsive, suspension time is short, the test score wassignificantly lower (P<0.01); test scores of the intervention group was significantly higherthan that of non-intervention group (P<0.01);②Modified of BBB results:14and21daysin the intervention group and non-intervention group scored significantly lower thannormal saline group (P<0.01); intervention group score significantly higher than non-intervention group (P<0.01);28days of age in the intervention group and the saline groupscore was no significant difference (P>0.05);non-intervention group scores weresignificantly lower saline group (P<0.01)4.Immunohistochemical results:Immunohistochemical analysis showed that all-day-old pups, LPS group offspring brain tissueMAG expression is significantly higher than the saline group (P <0.05,P <0.05), whichintervention group the expression of lower than non-intervention group (P<0.05).5. Stereologydetection results: in the saline group pups white matter the length of the myelinated nerve fiberdiameter was greater than that of non-intervention group and intervention group (P<0.05, P<0.05), and the group of intervention with the length of the nerve fiber diameter is greater thanthe non-intervention group (P<0.05).6.In LPS group the infant rat brain tissue MAG expressionwith its myelinated nerve fiber diameter length have no correlated.Conclusion:1. Intrauterine infection can lead to brain damage in offspring braintissue MAG expression of increased and its myelinated nerve fiber diameter is reduced inwhite matter.2. Early intervention and rehabilitation training can improve the functions ofthe movement which brain damage in offspring.3. Early intervention and rehabilitationtraining can inhibit the expression of MAG.4.Early intervention and rehabilitation trainingcan increate the myelinated nerve fiber diameter in the white matter of pups.5. Theexpression of MAG in LPS group rat brain tissue its change in diameter of the myelinatednerve fibers have not correlation.