Ling Gui Zhu Gan Tang Extract Against Myocardial Ischemia Pharmacodynamic Study

LingGuiZhuGanTang, a traditional formula from Shanghanlun, mainly used in the treatment of shortness of breath, fullness of chest, vertigo etc. due to the retention of fluid caused by yang-deficiency. Now it is often used as a remedy for cardiac bi-syndrome. The pharmacodynamics of anti-myocardial ischemia and anti-arrhythmia of LGZGT was studied in this paper.The results showed that LGZGT could apparently prolong survival time in normobaric hypoxia in mice; obviously alleviate acute myocardial ischemia induced by pituitrin and isoprenaline in rats. In addition, the activity of SOD is increased and the content of MDA is decreased in ischemic myocardium caused by isoprenaline. It was found that coronary blood flow and stroke amplitude were dramatically increased by drug-containing serum in isolated hearts of guinea-pig . Furthermore, it was also found that coronary vascular resistance (CVR), total peripheral vascular resistance(TPVR), heart rate (HR), systolic arterial pressure (SAP), left ventricular end diastolic pressure (LVEDP), oxygen utility rate, oxygen consumption index were all remarkably decreased whereas coronary blood flow(CBF) was markedly increased in canines. Moreover, the infract area dyed by NB-T was also distinctly reduced.LGZGT evidently protected mice from VF by chloroform. It also significantly antagonized arrthymias caused by calcium chloride, barium chloride and adrenaline. In hemorhelogy study, the data indicated that blood viscosity at high shear rate(HBV), blood viscosity at middle shear rate (MBV), blood viscosity at low shear rate (LBV), reduced blood viscosity(RBV), Erythrocyte aggregationindex(EAI), erythrocyte rigidity index(ERI), erythrocyte migration time(EMT) were obviously decreased.LGZGT remarkably inhibited thromobogenesis induced by collagen-adrenaline in mice and obviously inhibited thrombocyte aggregation induced by arachidonic acid(AA) in rabbits.LGZGT had duretic effect in rats and inhibited small intestinal transit in mice.Taken together, these results suggested that LGZGT was a promising recipe in the treatment of some myocardial ischemia and arrthymia.