Effect Of Anti-cardiac Muscle Lacking Blood Of Tranquilization Injection And Mechanism Study

Object:To discuss the effect and mechanism of anti-myocardial ischemia of Anxin injection,carry out its preclinical Pharmacodynamics studies,and to lay the foundation for development of the new clinical drug with effects of anti-myocardial ischemia and cardiovascular protection.Method:Rabbit's crown arteries were tied up near the first branch place in the from falling branch of the left coronary artery to establish the mode of myocardial ischemia.The scope of ischemia cardiac muscle was identified with the 0.1%NBT dyeing.Through applying the method of paraffin wax embedding,conventional slice and Iraq red dyes,the micromorphological change of myocardial infarction was observed.The standardⅡ-lead electrocardiogram was detected continuously by the needle electrode inserting under the hypodermis of limbs.The ascending aorta's blood stream was determined by Doppler method.The cardio hemodynamics was monitored through placing the pipe in the femoral artery to detect the HR,MAP,CO,LVSP,LVEDP and RPP.The CK and LDH in the blood are detected by automatic biochemistry analyzer.NO, SOD and MDA were determined by different colorimetric methods.The forming of thrombus in vitro was detected through the Chandler method. The aggregation ratio of platelet was determined under the Born nephelometry.Through using the LBY—NJ2 platelet aggregator,the platelet aggregation curve is drawn and the highest aggregation ratio is calculated,which can be used to analyze the effect of the drug on platelet aggregation.Blood Specific Viscosity,Blood Reduced Viscosity and Aggregation Index of RBC are determined respectively.The packed cellvolume is determined and the ESR is determined by Wintrobe method. The statistic software SPSS 11.0 is used to calculate the variance and analyze related data.Results:1.When the rabbit's crown artery was blocked for 60 min.,the infarction area of myocardial ischemia reached 29.4±3.7%in the model group.A mass of inflammation cells near the pathological cardiac muscle and hyperemia and dropsy were obviously observed under the microscope.They were also found that the cross striation and ridge cast of mitochondria come to be blurry,the matrix was light even to be bubbly and the cardiac muscle cells were taken on obvious aggregation, tumefaction,death and dissolution.Additionally,the typical characteristics of apototic cells,which were condensed nucleus,crinkled and abnormal karyotheca and coagulated chromatin,were also observed. All the observations mentioned above proved the successful modeling of myocardial ischemia.2.After the treatments with the low,medium and high dosage of Anxin injection,the cardiac muscle necrosis area was decreased to 25±3.2%,17±3.5%,14±2.9%,respectively.It was significantly different when compared with the model group.According to Micromorphology,it was observed that the inflammation and necrosis of cardiac muscle cells were markedly reduced,Which showed that the Anxin injection could reduce the cardiac muscle necrosis area and protect the ischemic cardiac muscle.The effect was better in the Anxin injection group with high dosage than that of the parallel group with glonoin.3.After the treatment with Anxin injection,the degree of the height and inversion of "S-T" phase and "T" phase in the rabbit's standard cardiogram dropped distinctly in contrast with the model group (P＜0.05～0.01),the percent of pathologic "Q" and ventricular arrhythmia declined remarkably(P＜0.05),which showed that the injection can remarkably improve the change of cardiogram of acute myocardial ischemia,slow HR,reduce the oxygen consumption of cardiac muscle and prevent arrhythrnia.4.After the treatment with Anxin injection,the rhythm of the ischemic heart became slow,and several indexes,such as MAP,CO, LVSP,RPP,were dropped while LVEDP was raised(P＜0.05).It showed that Anxin injection could improve the heart hemodynamics after being myocardial ischemia,and increase the supplementation of blood of cardiac muscle. 5.After the treatment with Anxin injection,CPK and LDH decreased obviously(P＜0.05).And the effect on CPK was better than that of the glonoine group while the effect on LDH was worse than that of the glonoine group.MDA had the marked decreasing and the SOD and NO had increasing(P＜0.05),which is distinctly different from the model group.(P＜0.01).It proved that the injection could improve the metabolism and the ability of anti-oxidization of ischemia cardiac muscle cell.6.After the treatment with Anxin injection,the length of the rabbit's thrombus was shortened obviously(P＜0.05)and the weight of thrombus was also listened.The Anxin injection can obviously reduce ADP and the ratio of platelet aggregation induced by either collagen or AA.It could also inhibit the Whole Blood Viscosity,Blood Specific Viscosity and Blood Reduced Viscosity of the rabbit in the trials,decrease the ESR and RBC,It showed that the effect of anti-myocardial ischemia of Anxin injection is related to its amelioration to the body's haemorheology.Conclusion:Anxin injection has good effect on anti-myocardial ischemia.The mechanism of its function might be related to that it can improve the index of the heart's hemodynamics and the metabolism and the anti- oxidization ability of ischemic cardiac muscle cell as well as inhibit the platetet aggregation and improve the hemorheology.