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Mainland China, The Wild Boar Population Genetic Diversity, Based Sandx-2 Sequence Variation And Sla-dqb Exon 2 Of The Pcr-sscp Analysis

Posted on:2007-02-24Degree:MasterType:Thesis
Country:ChinaCandidate:A L HuoFull Text:PDF
GTID:2190360185976909Subject:Zoology
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The wild boar (Sus scrofa), which inhabited in wide areas of Asia, Europe, and North Africa, is an ancestral Species of domestic pigs. In this paper, a review of single copy nuclear DNA (scnDNA) analyses in population genetic studies was given. Based on the analysis of SANDX-2 sequence and SLA-SQB PCR-SSCP pattern, the genetic variations of the Chinese wild boars were discussed.1. The anonymous nuclear DNA SANDX-2 locus of 89 individuals of Chinese wild boars and a domestic pig were amplified and sequenced for studying the genetic variability of Chinese wild boars. Total of 510 bp were obtained after aligned, including 4 indels and 30 substitutions. Eighteen haplotypes were defined in Chinese wild boars, among which 7 haplotypes were observed only in one local wild boar population, and 11 haplotypes were shared by two or more populations. The phylogenetic tree constructed by the neighbor-joining method based on Kimura 2-parameters model divided the haplotypes into two distinct clades, and it was not supported by high bootstrap value. The results indicated there was no significant geographical distribution pattern in the Chinese wild boars. Ignoring the gaps, the nesting cladogram of 19 haplotypes suggested that HAP2 may be ancestral haplotype.2. With the optimized PCR-SSCP protocol, exon 2 of SLA-DQB gene of 5 local wild boar populations was genotyped by PCR-SSCP methods. Total of 15 genotypes (AA, AB, BB, AC, AE, AH, BD, BF, BG, BH, DD, DG, DH, GG, EE) were observed in 5 local populations. There are 6 types (AB, AE, AA, AC, EE and BB) in Elunchun population, 4 types (AB, DH, BH and BB) in Hongxing population, 3 types (AB, BB and AA) in Guxian population, 7 types (BB, BD, BF, BG, DG, GG and DD) in Baoji population and 3 types (AB, BH and AH) in Jinzhai population. Gene B was shared by all the populations. Expected heterozygosity of the DQB alleles in each population ranged from 0.4444 to 0.7031, and the average was 0.6127. Our results also show that the exon 2 of SLA-DQB departure from Hardy-Weinberg Equilibrium in all wild boar populations, this may correlate with balancing selection in the MHC DQB alleles. Phylogenetic analysis indicated that there were closer relationship between Elunchun population and Jinzhai population, and among Hongxing population, Guxian population and Baoji population.
Keywords/Search Tags:Chinese wild boar, genetic divergence, SANDX-2, PCR-SSCP, SLA-DQB
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