Investigation On Macrofungi In Bayin Oboo National Nature Reserve And Studies On Taxonomy Of Laccaria In China

Macrofungi of Bayin Oboo National Nature Reserve, which is the extention of Great Khingan mountains, were investigated, and taxonomy of the genus Laccaria were studied too.A survey of macrofungi in Bayin Oboo National Nature Reserve was carried out during 2013-2014. A total of 184 species belonged to 2 phyla 4 classes 13 orders 32 families 74 genera were found. Among which, 82 species are edible, 46 species are medicinal, 36 species are poisonous and 78 species are ectomycorrhizal fungi. Specimens are deposited in the Herbarium of Mycology of Jilin Agricultural University(HMJAU). The habit and utilize of each species were also recorded. In addition, 5 species, Conocybe macrospora(G.F. Atk.) Hauskn., Cortinarius casimiri(Velen.) Huijsman, C. diasemospermus Lamoure, C. vernus H. Lindstr. & Melot and Inocybe fuscidula Velen, are new to China, 32 species are new to Inner Mongolia. And the macrofungi resources utilization of Bayin Oboo Nature Reserve was evaluated briefly.195 Laccaria specimens which were deposited in Herbarium, Kunming Institute of Botany, CAS(KUN), Herbarium of Mycology of Jilin Agricultural University(HMJAU) and collected by our team were studied both in macro- and micro- morphology. 18 species were conducted to the morphological descriptions, illustrations, line drawings and identification key. Among which, 3 species, L. longipes G.M. Muell., L. amethysteo-occidentalis G.M. Muell. and L. trichodermophora G.M. Muell., were new to China. The ITS of 9 Laccaria species were sequenced and phylogenetic tree was constructed by Maximum Likelihood Method. Result showed that similarity of 9 sequences compared with data from Genbank are more than 98%, and also the 9 sequences are clustered in same clade separately, which support morphological result. The Laccaria has a closer relationship with Hydnangium than Tricholoma, so Hydnangiaceae includes Laccaria is supported. The basidiospores of Laccaria species were studied by SEM. Result showed that spores of Laccaria are globose to broadly ellipsoid with obvious echinulate.