The Design And Fabrication Of Chip-based Capillary Electrophoresis By UV-LIGA Technology

At present, chip-based capillary electrophoresis has already surmounted the bottlenecks on it's fabrication technology and basic fluidic technology which it met at the beginning. Instead, it's coming into a new epoch of deepening it's fundamental reseach, expanding it's applied realm and realizing it's deeper industrialization. Therefore, we must find an applied way to make it. UV-LIGA technology provides more room for the development of chip-based capillary electrophoresis, for the advantages on it's high fabrication precision,being prone to mass production and lower price.The work presented in this thesis consists of four sections. The first section is the implementation of microelectroforming system, the second is the design and fabrication of chip-based capillary electrophoresis by UV-LIGA technology, the third is the analysis of electrolyte, and the last section is the verification of the chip-based capillary electrophoresis system in biology.With the resources we had, we designed and implemented a microelectroforming system, then optimized the parameters and fulfilled the fabrication of capillary electrophoresis microfluidic chip with it successfully. We also put forward creatively a low-melting-point-metal-aided demolding process to crack the hard nut on the demolding of mandrel core based on silicon chip. It has been proved that this method can enhance the efficiency of demolding, simplify the process, do good to protecting the micro structure and enhance the yield.We also introduced spectrophotometer into the analysis of sulfamate type electrolete to measure the content of nickel sulfamate for the first time. This method is much easier than the conventional chemistry titration, it can be accomplished within one minute. Furthermore, it enhances significantly the precision of the measurement. And at the same time, the consumption of the sample is only one thousandth contrasted with the former method.At last, we fulfilled the experiment on the analysis of the lenth of DNA segment which testified that the efficiency of the separation was satisfying.