Set Up Of Pretreatment Unit By Solid Phase Extraction In Microfluidic Analytical System

Due to Miniaturized total analytical system (μ-TAS) be prone to realize analysis process integration, automatization and micromation on ship, thus it can greatly reduce reagent wastage, shorten analysis time and improve testing efficiency, which make it paid universal attention in fields of disease diagnoses, biochemical analysis, clinic test and so on. One of effectual approaches to realize integration is using chips with different configuration and function to constitute the whole analysis process. Aim at complex biochemical system; sample pretreatment is precondition to actualizeμ-TAS analysis, presently, the pretreatment technology become bottleneck that must be broken through for integration, great efficiency and concatenation of developingμ-TAS.Base on summarization separateness enrichment technologies internal and overseas in micro-fluidic analytical system, silica monolithic column emerging from chromatogram realm was made use of solid-phase extraction medium material inμ-TAS in our research. Firstly, silica monolithic column with double hole was prepared by sol-gel technique, then via modified with esterified group, C18 esterified silica monolithic column was obtained. Further, its framework and speciality was charactered by some methods such as scan electron microscope (SEM) and infrared spectrum (IR). Combining the requirement ofμ-TAS analytical system and the characteristic of silica monolithic column, simple solid-phase extraction pretreatment unit was set up; subsequently its pretreatment efficiency also was tested by optimal Dobutamine Hydrochloride-KMnO4-Luminol chemiluminescence system. The results showed that column content of C18 esterified silica monolithic column to Dobutamine Hydrochloride was 0.0198μg·mg-1, and enrichment multiple can reach 95. Further more, trace Dobutamine Hydrochloride in serum was enrichment by C18 esterified silica monolithic column, from the testing, average enrichment multiple was get 85, RSD get 11.79%. Apparently, the kind of pretreatment unit was short of conformable precision, which ascribing to large molecule stop up C18 esterified silica monolithic column to decrease recurrence. Therefore, affinity membrane was introduced into pretreatment unit to get rid of interferer from large molecule such as albumin in serum and to improve recurrence. Polyamide membrane was selected as basic material to further modify by Cibacron Blue F3GA in order to adsorb albumin selectivity. Affinity membrane combined with micro-fluidic chip by inbuilt and external methods, and external-combination was choosed finally from the result of optimal Dobutamine Hydrochloride-KMnO4-Luminol chemiluminescence system. Then C18 esterified silica monolithic column-affinity membrane-microchip pretreatment unit was set up. Using this pretreatment unit, trace Dobutamine Hydrochloride in serum was enrichment by C18 esterified silica monolithic column, and average enrichment multiple was get 89.36, RSD get 4.37%. The result indicated that feasibility and validity of the pretreatment unit for enrichment trace medicament in serum.