The Study Of IL-6 And IL-12P70 And β-Endorphin And Histamine In Serum Of Patients With Chronic Idiopathic Urticaria

Objective:This study determined the levels of interleukin-6 (IL-6), interleukin-12P70 (IL-12P70),β-Endorphin and histamine to investigate the status of nerve-mental in the pathogenesis of CIU.Method:62 patients with CIU (including 35 patients with lesion and 27 patients without lesion when their blood samples were collected) and 34 healthy controls were selected at random. The serum levels of IL-6 and IL-12P70 and histamine andβ-Endorphin were measured in 62 patients and 34 healthy controls with double antibody sandwich enzyme-linked immuno-sorbant assay (ELISA) method. The results were analyzed by statistical package for the social science17.0 (SPSS17.0).Results:①The level of IL-6 in the groups of CIU patients with lesion, CIU patients without lesion and healthy controls were 458.19±72.79pg/ml,408.19±68.79pg/ml,432.32±92.38 pg/ml, and the level of IL-6 in the three groups showed no statistically significant difference (p>0.05).②The level of IL-12P70 in the groups of CIU patients with lesion, CIU patients without lesion and healthy controls were 286.39±138.06 pg/ml,328.39±112.96 pg/ml,366.76±115.82pg/ml. The level of IL-12P70 in the three groups showed statistically significant difference (p<0.05), the level of IL-12P70 in CIU patients with lesion showed lower than that of healthy controls (p<0.05). But there was no statistically significant difference between CIU patients without lesion and healthy control (p>0.05), and there was no statistically significant difference between patients with lesion and without lesion (p>0.05).③The level ofβ-endorphin in the group of CIU patients with lesion, CIU patients without lesion and healthy controls were 19.42±5.93ng/ml,18.70±6.12ng/ml,15.70±4.92ng/ml, and the level ofβ-endorphin in the three groups showed statistically significant difference (p<0.05). The level ofβ-endorphin in CIU patients with lesion were significantly higher than those in healthy controls (p<0.05). The level ofβ-endorphin in CIU patients without lesion were significantly higher than those in healthy controls (p<0.05). But there was no statistically significant difference between CIU patients with and without lesion (p>0.05).④The level of histamine in the group of CIU patients with lesion, CIU patients without lesion and healthy controls were 34.03±14.32ng/ml,27.70±9.32ng/ml,20.61±10.71ng/ml, and the level of histamine in the three groups showed statistically significant difference (p<0.05). The level of histamine in CIU patients with lesion were significantly higher than those in healthy controls (p<0.01). The level of histamine in CIU patients without lesion were significantly higher than those in healthy controls (p<0.05). The level of histamine in CIU patients with lesion were significantly higher than CIU patients without lesion (p<0.05).⑤The level of histamine has positive correlation with the level ofβ-endorphin in CIU patients with lesion (r=0.712, p<0.01). The level of histamine has negative correlation with the level of IL-12P70 in CIU patients with lesion (r=-0.357, p<0.05). The level ofβ-endorphin has no correlation with the level of IL-12P70 in CIU patients with lesion (r=0.167, p>0.05). The level of histamine has positive correlation withβ-endorphin in CIU patients without lesion (r=0.615, p<0.05). There were no significant correlation in CIU patients without lesion were found between the levels of histamine and IL-12P70 (r=0.128, p>0.05). There were no significant correlation in CIU patients without lesion were found between the levels ofβ-endorphin and IL-12P70 (r=0.147, p>0.05).Conclusions:①The level of IL-12P70 declined in CIU patients, the results suggest that disbalance of the T1/T2 immunology may play a role in pathogenesis of CIU.②Histamine plays a role in the pathogenesis of chronic idiopathic urticaria.③Histamine maybe take part in disbalance of the T1/T2 immunology, can restrain cell immune then initiate and (or) aggravate development of CIU.④The level ofβ-endorphin increased in CIU patients, the result sugust that neuogenic factors maybe take part in the pathology of CIU.⑤Neuogenic factors may be initiate and (or) aggravate development of CIU through stimulating mastocyte degranulation then releasing histamine and inducing disbalance of the T1/T2 immunology.