Expression Of YKL-40 And NF-κB In Endometrial Carcinoma And Their Correlation

Objectives:To detect the expression of YKL-40 and nuclear factor-kappaB(NF-κB) in endometrial cancer,to analyze their relationship with clinical-pathologic parameters and their expressed relationship in endometrial cancer,thereby investigate the role of YKL-40 and NF-κB in the carcinogenesis and development of endometrial carcinoma initially.Methods:Immunohistochemistry was utilized to measure levels of expression of YKL-40 and NF-κB/P65 in the endometrial tissue specimens ,The endometrial tissue specimens were obtained from 65 cases with endometrial carcinoma,20 cases endometrium with proliferative phase and 20 cases endometrium with secretory phase.Results:All of the endometrium tissues include normal endometrium and endometrial carcinoma were scored as YKL-40,The positive expression of YKL-40 in normal endometrium was lower significantly than tissues of endometrial carcinoma (32.5% ,76.92%, P=0.000,P<0.01) .The positive expression of YKL-40 in normal proliferative phase of endometrium and in secretory phase of endometrium has no statistic difference (30% ,35%, P=0.736,P>0.05) .The percentage of YKL-40 positivity in moderately and poorly differentiated tissues was higher than that in well differentiated endometrial carcinoma tissues (90%,65.71%,P=0.021,P<0.05).YKL-40 expression did not correlate with any other clinical pathologic parameters such as FIGO stage ,myometrial invasion or lymph node status(P=0.561,P=0.456,P=0.623,P>0.05).The expression of NF-κB/P65 were scored in normal endometrium and in tissues of endometrial carcinoma.NF-κB/P65 expression was lower significantly in normal endometrium than that in carcinoma tissues(30% , 73.85% , P=0.000 ,P<0.01).It's expression ratio of positive was 35% in normal proliferative phase of endometrium and was 25% in secretory phase of endometrium,There was no statistic difference between them(P=0.490,P>0.05).The percentage of NF-κB/P65 positivity was higher in moderately and poorly differentiated tissues than that in well differentiated endometrial carcinoma tissues (86.67%,62.86%, P<0.05).There was no relationship between expression NF-κB/P65 expression with FIGO stage, myometiral invasion,or lymph node status(P=0.099 ,P=0.08, P=0.485, P>0.05).There was no statistical difference between YKL-40 and NF-κB/P65 in endometrial carcinoma(P=0.687,P>0.05)by Mc Neman's Test,At the same time,there was a positive correlation between YKL-40 and NF-κB/P65 expression in endometrial carcinoma tissues(r=0.754,P=0.000,P<0.01) by the analytical method of Spearman.Conclusion:1 : Expression of YKL-40 showed an abnormal increasing in endometrial carcinoma compared to normal endometrial,and higher significantly in moderately and poorly differentiated endometrial carcinoma tissues than that in well differentiated tissues,which suggested up-regulation of YKL-40 may be play important role in the tumorigenesis and development of endometrial carcinoma.2: NF-κB/P65 expression was lower in normal endometrial compared to carcinoma tissues,and higher significantly in moderately and poorly differentiated endometrial carcinoma tissues than that in well differentiated tissues,which suggested up-regulation of NF-κB/P65 may be play important role in the tumorigenesis and development of endometrial carcinoma.3:Both NF-κB/P65 and YKL-40 showed an abnormal increasing tendency in endometrial carcinoma from well-differentiated to moderately and poorly differentiated endometrial carcinoma,there was a positive correlation between YKL and NF-κB /P65 expression in endometrial carcinoma tissues,further study about NF-κB,YKL-40,oncogenes,anti-oncogene,related genes should be performed to elucidate the precise role of NF-κB and YKL-40 in the initiation and promotion of endometrial carcinoma.