Part 1 The Influence of Qi-Supplementing and Yin-Nourishing recipe on Th2 Shift in elderly patients with non-small-cell lung cancerã€OBJECTIVE】To investigate the effect of chemotherapy and Qi-Supplementing and Yin-Nourishing recipe on Th1/Th2 shift in elderly patients with non-small-cell lung cancer.ã€METHODS】Sixty patients were randomly divided into three groups: Chinese herbal medicine group,chemotherapy combined with Chinese herbal medicine group and chemotherapy group. After two treatment courses, serum levels of IFN-γ,IL-2,IL-4 and IL-10 were assessed by ELISA, using IFN-γand IL-2 as Th1 type cytokines,IL-4 and IL-10 as Th2 type cytokines.ã€RESULTS】After chemotherapy, the levels of IFN-γand IL-2 were significantly higher, while thelevels of IL-4 and IL-10 were significantly lower. Chemotherapy combined with Chinese herbal medicine significantly reduced the levels of IL-4 and IL-10 and in Chinese herbal medicine group the levels of IFN-γand IL-2 were obviously increased. For chemotherapy group, there were higher levels of Th1 type cytokines and lower levels of Th2 type cytokines in combination group and Chinese herbal medicine group.ã€CONCLUSION】The results show that chemotherapy would contribute to the growth of Th2 type cytokines and the Qi-Supplementing and Yin-Nourishing therapy could reverse Th1/Th2 shift in elderly patients with non-small-cell lung cancer. Part 2 Molecular Mechanism of Qi-Supplementing and Yin-Nourishing recipe on Th2 Shift in elderly patients with non-small-cell lung cancerã€OBJECTIVE】This study was carried out to explore the possible molecular mechanism of Qi-Supplementing and Yin-Nourishing recipe on Th2 Shift in elderly patients with non-small-cell lung cancer.ã€METHODS】Sixty patients were randomly divided into three groups: Chinese herbal medicine group,chemotherapy combined with Chinese herbal medicine group and chemotherapy group. After two treatment courses, the expression of T-bet mRNA and GATA3 mRNA of peripheral blood mononuclear cells(PBMC) were detected by RT-PCR, using T-bet as the transcription factor of Th1 cell and GATA3 as the transcription factor of Th2 Cell.ã€RESULTS】The gene expression of T-bet mRNA in Chinese herbal medicine group and chemotherapy combined with Chinese herbal medicine group was obviously higher than that of chemotherapy group. Simultaneously, the gene expression of GATA-3 mRNA in Chinese herbal medicine group and chemotherapy combined with Chinese herbal medicine group was significantly lower than that of chemotherapy group.ã€CONCLUSION】The results show that the Qi-Supplementing and Yin-Nourishing therapy could reverse Th1/Th2 shift in elderly patients with non-small-cell lung cancer and its molecular mechanism might be that the Qi-Supplementing and Yin-Nourishing therapy could regulate the expression of transcription factors T-bet/GATA3 to control a complicated molecular network composed by cytokines,transcription factors and cellular signal transduction pathway.
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