The Expression And Significance Of Soluble Endoglin And Soluble Fms-like Tyrosine Kinase In Preeclampsia

ObjectivesTo discuss the serum levels and significance of soluble endoglin and soluble fms-like tyrosine kinase in women with preeclampsia and their relationships.MethodsForty-one preeclampsia patients in GuangZhou women and childrens'medical center from Nov.2008 to Nov.2009 were divided into two groups: mild preeclampsia group and severe preeclampsia group. Mild preeclampsia group included 15 cases of mild preeclampsia,with the mean gestational age at sampling of 34.1±2.1,the mean maternal age of 29.9±6.4 and the mean BMI(23.4±3.7) kg/m2.Severe preeclampsiagroup included 26 cases,with the mean gestational age at sampling of 33.1±4.9,the mean maternal age of 31.6±3.7 and the mean BMI(23.5±5.9) kg/m2.The control group was composed of 40 cases of normal pregnant women during the same period,with the mean gestational age at sampling of 34.9±3.6,the mean maternal age of 30.4±3.6 and the mean BMI(22.2±4.9) kg/m2.The ELISA method was used to detect the concentrations of soluble endoglin and soluble fms-like tyrosine kinase.Parametric statistics were used for data analysis.Results 1. The concentration of soluble endoglin(sEng) from serum samples:1)The mean serum sEng level in the control group was (2.59±0.64) ng/mL, and (4.42±1.12) ng/mL in the mild preeclampsia group, (5.79±0.98) ng/mL in the severe preeclampsia group. And the data showed obvious difference with variance analysis(P<0.05).2)The serum sEng level in mild preeclampsia group was significantly higher than those in control group [ (4.42±1.12)ng/mL VS. (2.59±0.64)ng/mL, P<0.05].3)The serum sEng level in severe preeclampsia group was significantly higher than those in control group [ (5.79±0.98)ng/mL VS. (2.59±0.64) ng/mL, P<0.05].4)The serum sEng level in severe preeclampsia group was significantly higher than those in mild group [ (5.79±0.98)ng/mL VS. (4.42±1.12) ng/mL, P<0.05].2.The concentration of soluble fms-like tyrosine kinase (sFlt-1) from serum samples:1) The mean serum sFlt-1 level in the control group was (568.7±172.3) ng/L, and (2393.6±620.2) ng/L in the mild preeclampsia group, (2481.8±934.3) ng/L in the severe preeclampsia group. And the data showed obvious difference with variance analysis(P<0.05).2) The serum sFlt-1 level in mild preeclampsia group was significantly higher than those in control group [ (2393.6±620.2) ng/L VS. (568.7±172.3)ng/L, P<0.05].3) The serum sFlt-1 level of severe preeclampsia group was significantly higher than those in control group [ (2481.8±934.3) ng/L VS. (568.7±172.3)ng/L, P<0.05].4) The serum sFlt-1 level in severe preeclampsia group did not differ from the mild group [ (2481.8±934.3)ng/L VS.( 2393.6±620.2)ng/L, P>0.05].3.FGR sub group VS. non-FGR sub group1) The serum sEng level of FGR sub group was significantly higher than those of the non-FGR sub group [ (6.26±0.71)ng/mL VS. (4.84±1.16)ng/mL, P<0.05] in the preeclampsia group.2) The serum sFlt-1 level of FGR sub group was significantly higher than those of the non-FGR sub group [ (3159.9±655.1)ng/L VS. (2167.9±699.1)ng/L, P<0.05] in the preeclampsia group.4.The Correlation between the serum levels of sFlt-1 and sEng and MAP in severe preeclampsia.1) There was a significant positive correlation between the serum levels of sEng and MAP (r =0.571,P<0.05).2) There was no significant correlation between the serum levels of sFlt-1 and MAP (r =0.238,P>0.05).5. The Correlation between the serum levels of sFlt-1 and sEng and proteinuria in severe preeclampsia.1) There was no significant correlation between the serum levels of sEng and proteinuria (r =0.216,P>0.05).2) There was a significant positive correlation between the serum levels of sFlt-1 and proteinuria (r =0.488,P<0.05).6.Correlation analysis: There was a significant positive correlation between the serum levels of sFlt-1 and sEng (r =0.570,P<0.05)Conclusions1.The serum sEng level increases in preeclampsia, and has correlation with disease severity.2.The serum sFlt-1 level increases in preeclampsia, and has no correlation with disease severity.3.The serum sEng and sFlt-1 level in preeclampsia has correlation with FGR.4. sFlt-1 and sEng are involved in the pathogenesis of preeclampsia with cooperative, but different roles.5.sEng and sFlt-1 have a synergy effect during the preeclampsia.