Expression Of Urinary Soluble Fms-like Tyrosine Kinase-1 With Preeclampsia

Objective: Pre-eclampsia is a specific pathologic process of gestation period.Although amount of reseach have done about it,the etiology and pathogenesis of pre-eclampsia remains unclear.Clinical diagnostic methods are monitoring of blood pressure, analysis of urine protein, test of serum uric acid,but the specificity and sensitivity are not optimal.Update,the study showed that the imbalance between angiogenesis factor and antiangiogenic agent is the important reason.Among them,it focuses on soluble fms-like tyrosine kinase-1 (sFlt-1)generated by placenta.sFlt-1 is inhibit receptor of vascular endothelial growth factor (VEGF). It can inhibit the physiologic action of VEGF and placental growth factor (PlGF) by combined them.It was recently shown that concentration of sFlt-1 in placenta and blood of patients is high.So far the study is concentrated on the variety of sFlt-1,VEGF and PlGF in serum.And it is found that there exists singnifi– cant difference of sFlt-1 in serum between pre-eclampsia and normal pregnant.Meanwhile sFlt-1 indentify the degree of the disease. However,in our experiment, we sought to the possibly change of sFlt-1 in urine and the action of it.At the same time, in order to find out an noninvasive method,an experimental diagnostic mark with high specificity and sensitivity,we compared sFlt-1 with other clinical indexes.Methods:Gathering the serum of 36 patients in pre-eclampsia without complications and using the drugs affecting the vacular activity, severe pre-eclampsia ,non severe pre-eclampsia and normal pregnant are 16,13and15 respectively.sFlt-1 is measured by ELISA,urine protein by biuret colorimetry, serum uric acid by phosphotungstic acid. Results: (1)In severe pre-eclampsia,non severe pre-eclampsia and normal pregnant groups, the quanlity of urine protein is 2.88,1.31 and 0.37 respectively, the quanlity of uric acid in serum is 380,312 and 209umol/L respectively (P=0.004),There exists singnificantly difference(P<0.05). (2)the quanlity of sFlt-1 is 2986.25,2189.38 and 1231.60mg/ml respectively(P=0.000).There exists singnificantly difference (P<0.05). (3)Among them,there exist correlation ship.The r between urine protein and uric acid,urine protein and sFlt-1,uric acid and sFlt-1 is 0.582 and 0.325 respectively (P<0.05).(4)From the ROC curve,we can see that the specificity and sensitivity of sFlt-1 is the highest.Conclusion: (1) sFlt-1 plays a important role in the creation of pre- eclampsia.And sFlt-1 indentify the degree of the disease.(2)Evaluation of sFlt-1 in urine is an noninvasive and quick meathod.The specificity and sensitivity of sFlt-1 are the much highest than another to identify the degree of severe pre-eclampsia.(3) sFlt-1 in casual urine has positive singnificance while larger than 2676mg/ml.