Strong Bone Pain Extracts On The Efficacy Of Osteoporosis And The Mechanism Of Female

Objective To verify the Strong bone pain extracts on the efficacy of osteoporosis in female mice and to screen the effective area, Zhuanggu prescription painkillers for further study lay the foundation for an effective monomer.Methods Experiments with female rats to go after the establishment of osteoporosis model,150 SD female rats With a 3 month old (250g±), Randomly divided into normal group, model group, sham operation group, strong bone pain side extraction group, Group A water extraction, water extraction B, A group of ethanol extract, ethanol extract B, ethyl acetate extract A Group A group of petroleum ether extract, total of 10 groups, N=15, In addition to the normal group, sham operation group, The remaining groups were ovariectomized (removal of both ovaries) and then randomly divided into eight groups, Week after administration, After 13 weeks of continuous administration, were sacrificed blood, bone tissue for estrogen, BGP, serum PTH, CT and bone mineral density, bone biomechanics (maximum stress, maximum bending strength) pathology, ultrastructure, and many other bone Observation.Results 1.strong bone pain side extraction group and the group can effectively extracts of osteoporosis in ovariectomized female rats increased the femur and lumbar spine bone mineral content (P<0.05 or P <0.01), can effectively prevent osteoporosis Disease; 2. strong bone pain side extraction group and the group can effectively extracts of E2 level significantly increased (P<0.01) reduced the levels of BGP (P<0.01), it may be by promoting the secretion of estrogen, regulate BGP to reduce the rate of bone turnover and reduce bone loss, in order to achieve the role of anti-osteoporosis; 3. strong bone pain side extraction group and the group can effectively extract parts can reduce the level of serum PTH (P<0.05), the levels of serum CT no significant effect (P> 0.05), probably by reducing PTH Inhibit bone resorption, in order to achieve the efficacy of anti-osteoporosis.4.strong bone pain side extraction group and the group can effectively improve the extracts of the maximum tibial stress and maximum bending strength (p<0.05 or p<0.01), the tibia bone by increasing bone strength, can improve osteoporosis Disease.5. strong bone pain side extraction group and the group can effectively increase the extracts of the uterus in ovariectomized female wet weight (p<0.05 or p <0.01); 6. strong bone pain side group and its effective parts extract extract can increase trabecular bone density group (p<0.05 or p<0.01), can reverse osteoporosis, pathological changes.7. strong bone pain side extraction group and the group effectively extracts of osteoporosis can improve bone cells within the nucleus, mitochondria, cell processes such as ultrastructure.8. A group of petroleum ether extract, water extract B, A group for the ethanol extract Zhuanggu analgesic efficacy party site.Conclusion A group of petroleum ether extract, water extract B, A group for the ethanol extract Zhuanggu analgesic efficacy party site.By increasing the level of estrogen, lower bone turnover, inhibit the conversion of osteoclasts, reducing bone resorption, increase bone formation, increase bone density and improve the molecular structure of bone matrix to enhance osteoporosis in ovariectomized female mice Biomechanical properties to prevent and treat osteoporosis.