| Objects:This study aims to explore the intrinsic mechanism of postmenopausal women of serum IL-8,10levels and postmenopausal women with osteoporosis disease. Further explore the new mechanism of osteoporosis, put forward new ideas for clinical prevention and treatment of postmenopausal osteoporosis.Methods:The first part:Serum cytokine detection in postmenopausal women: Subjects were fasting for more than12h, Venous blood5ml, and the serum was separated, detects the following indicators:Dual-energy X-ray absorptiometry to measure bone mineral density, measured by radioimmunoassay of serum IL-8, IL-10level. The second part:Construction the rat model of osteoporosis and serum cytokine detection,306-month-old female SD rats, adaptive feeding after two weeks in the experimental Animal Center of Tianjin Municipal People’s Hospital were randomly divided into three groups:normal control group (Sham), model group (OVX), estradiol (OVX+estradiol). Model group and estradiol group was established by ovariectomy model of osteoporosis. Normal control group the same way laparotomy, but not ovariectomy, ovariectomy next to the same size of adipose tissue. From the third week after surgery intragastric administration for the each group Rats. Estradiol valerate with distilled water into a0.01%suspension, the estradiol valerate group to give1mg/kg/d, normal control group and model group were given the same volume of distilled water. Animals were sacrificed after12weeks, separating the serum and determing of in vivo bone mineral density in rats, detecting the following indicators: Determination of Body, lumbar spine, both sides femoral bone mineral density by dual energy X-ray absorptiometry (BMD), measured by radioimmunoassay of serum IL-8, IL-10level.Result:1. Serum IL-8levels in rats after castration compared with the sham group, the tendency increased, IL-8levels were significantly decreased after estradiol valerate treatment, has no significant difference with the sham group. In ovariectomized rats decreased serum IL-10levels compared with the sham group, IL-10levels increased significantly after estradiol valerate treatment, has no significant difference with the sham group.2. Model rats BMD was significantly lower than other groups, BMD was significantly improved after estradiol valerate treatment, has no significant differences with the sham group.3. Postmenopausal women with osteoporosis serum IL-8level and no osteoporosis in postmenopausal women have more tendency to increase, but the serum IL-10levels in postmenopausal women with osteoporosis than postmenopausal women with no osteoporosis have decreased.4. The serum IL-8expression level in rats and postmenopausal women was positively correlated with the degree of osteoporosis, Serum IL-10expression level was negatively correlated with the degree of osteoporosis.Conclusion:1. Estradiol valerate significantly enhanced the bone mineral density of ovariectomized rats, reduced serum IL-8levels, elevated serum IL-10levels. The serum IL-8levels and osteoporosis disease is positively correlated, the serum IL-10levels and osteoporosis disease is negetively correlated.2. Postmenopausal women with osteoporosis serum IL-8levels and osteoporosis disease is positively correlated, Postmenopausal women with osteoporosis serum IL-10levels and osteoporosis disease is negetively correlated.3. Serum IL-8, IL-10levels are likely to become one of the indicators for diagnosing osteoporosis, Serum IL-10levels may be one of the indicators of the evaluation of the efficacy of osteoporosis. Adjusting the level of serum interleukin may become a new method for the treatment of osteoporosis. |
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