Association Of HLA-DQB1,DPB1 Alleles With Polymorphous Light Eruption Of The Han Nationality In Yunnan

objective To explore the relationship between the allele of HLA-DQB1,DPB1 and polymorphous light eruption of the Han Nationality in Yunnan.Method:Classical phenol-chloroform method was applied to extract DNA from vein blood of 38 PLE patients and 45 healthy people,then PCR-SBT was used to examine the HLA-DQB1,DPB1 allele gene of the people mentioned above.Result:All sequence results of PCR product of 38 PLE patients and 45 control can be find the relevant gene type in the latest HLA-DQB1,DPB1 sequence.Statistics of their frequency,relative risk frequency indicate that the frequency of HLA-DQB1*050301 in PLE is higher than in control,and diversity is remarkable(x~2=5.691,P＜0.05),but the rest has no significance.Conclusion:Inferring that HLA-DQB1*050301 may be the susceptible gene or linkage disequilibrium with the real susceptible gene of PLE of the Han Nationality in Yunnan.No susceptible gene or protective gene of HLA-DPB1 was discovered,and maybe that HLA- DPB1 has no association with PLE.