Expression And Significance Of HIPK₂ In The Tissues Of Cervix Carcinoma And Cervical Intraepithelial Neoplasia

Objective: To study the relationgship between homeodomain-interacting protein kinase 2 (HIPK2) and occurrence and development of cervical cancer in order to provide a basis for early diagnosis of cervical cancer through investigrating the expression of HIPK2 and its relevance with apoptosis in cervical cancer and precancerous lesion tissues.Methods:The immunohistochemical staining and RT-PCR were used to assay the HIPK2 expression in 20 cases of cervical cancer tissues, precancerous lesions (12 cases of CINâ…¢and CINâ… -â…¡tissues,respectively) and 10 cases of normal cervical tissues to investigate the protein and mRNA expression of HIPK2.P53 expression and apoptosis of precancerous lesions (12 cases of CINâ…¢and CINâ… -â…¡tissues,respectively) and 10 cases of normal cervical tissues were detected by immunohistochemistry and TUNEL,respectively.Results: (1) Immunohistochemistry results showed that the HIPK2 expression was gradually increased in the cervical cancer, CINâ…¢,CINâ… -â…¡and normal cervical tissues. (2) P53 protein expression was negative in normal cervical epithelial cells and positive in abnormal squamous cells of the CIN group.(3) There were a small number of apoptotic cells in normal cervical epithelial cells and nearly none in abnormal squamous cells of the CIN group (TUNEL).(4) RT-PCR results also showed a gradually increasing trend of the HIPK2 mRNA expression from cervical cancer, CINâ…¢, CINâ… -â…¡to the normal cervical tissues.And the differences of HIPK2 expression between in cervical cancer group and in the other groups were statistically significant (P<0.05). There were also statistically significant differences of HIPK2 expression between in CINâ…¢group and in the normal cervical group (P<0.05). And the expression differences of HIPK2 in CINâ… -â…¡and normal cervical group were not statistically significant (P>0.05). (5) The expression of HIPK2 was negatively correlated with p53 expression(r=-0.453,P<0.05) and positively correlated with cell apoptosis (r=0.393,P<0.05) in the cervical cancer, CINâ…¢,CINâ… -â…¡and normal cervical tissues.Conclusion: The reduced expression of HIPK2 and overexpression of P53 might play an important role in the cervical carcinogenesis and progress.And combined detection of HIPK2 and p53 might contribute to the pathogenesis study of cervical precancerous lesions and cervical cancer and improve their diagnostic rate.