Study On Resisting Lipid Peroxidation Of Pigment From Maize Purple Plant

Objective: Anthocyanin was a kind of water-soluble natural pigment that derived from various edible plants and has antioxidative activity. Anthocyanin was recognized as functional food factors. Pigment from maize purple plant (PMPP) was a kind of anthocyanins pigment. PMPP was red color that was extracted from a new kind of maize. The complete structures of PMPP anthocyanins were elucidated as cyanidin-3-glucoside. So far it has not been reported the effect of antioxidant activity in PMPP. In this study,we examined the antoxidant effects of PMPP on inhibiting lipid peroxidation. Method: (1) In autcoxudation of lecithin liposome system induced by Fe2+,the inhibition ratio of PMPP in concentration as 0.1mg/ml,0.3mg/ml,0.5mg/ml,0.7mg/ml,0.9mg/ml on linoleic acid oxidation were measured using TBA respectively. Control was ascorbic acids.(2) Effect on the lipid peroxidation of liver homogenate in vivo. After 4 Kunming rats were dead, whole liver was removed and made hepatic homogenate which was composed of 10 volumes (wt/v). The inhibition ratio of PMPP in concentration as 0.1mg/ml,0.3mg/ml,0.5mg/ml,0.7mg/ml,0.9mg/ml on linoleic acid oxidation were measured in hepatic homogenate using TBA respectively. Control was ascorbic acids.(3)Protective effect of PMPP on bromobenzene-induced oxidative liver damage of miceAccording to the method for the Assessment of antioxidative function in"Technical standards for test & assessment of health food"issued by Healthy Ministry of PRC, the protective effect of maize purple plant pigment on bromobenzene-induced liver damage of mice was test. 50 Kunming mice were divided randomly into five groups. They were intragastric administ- ration (i,g) 10ml/(kg.bw) of vehicle and different doses (control group, injury control group, low group: 5.0g/L, middle group: 10.0g/L,high group: 30.0g/L) for 30 days. One hour passed after fasting rats were intragastric administration PMPP , All rats were gave 10ml/(kg.bw) of 3mmol/L bromobenzene by i.g. except control group. After 22 hours, Blood was collected from the vena cava and whole liver was removed. The concentration of malonaldehyde(MDA) and superoxide dismutes(SOD) activities in blood serum and liver were determined respectively.Result: (1) The inhibition ratio of PMPP on linoleic acid oxidation was higher that of ascorbic acids in autcoxudation of lecithin liposome system induced by Fe2+. The inhibition ratio of 0.1mg/ml PMMP was 34.2%. The inhibition ratio of 0.1mg/ml ascorbic acids was 18%. When the inhibition ratio was 60%, the concentration of PMPP and ascorbic acids were 0.9mg/ml and 5mg/ml, respectively.(2) PMPP inhibited lipid peroxidation in hepatic homogenate of rats. The inhibition ratio of 0.1mg/ml PMMP was 36.3%. The inhibition ratio of 0.1mg/ml ascorbic acids was 5.5%. When the inhibition ratio was 80%, the concentration of PMPP and ascorbic acids were 1 mg/ml and 10mg/ml, respectively.(3) Protective effect of PMPP on bromobenzene-induced oxidative liver damage of miceThe concentration of MDA in liver in Middle and high dose of pigment were significantly lower than that in low dose and the injure control group(t =4.867,P=0.0448;t = 6.838,P=0.0142 ). There were no significant differences in the concentration of MDA in liver between low dose group and injury control group. There was no significant difference in SOD activity in liver among groups. There were no significant differences in the concentration of MDA and SOD activity in serum.Conclusion:The PMPP had strong the capability to resist lipid peroxide.