| IntroductionEndemic fluorosis is widely epidemical in the world. China is one of the countries with severest endemic fluorosis. Almost all provinces but Shanghai and Hainan have fluorosis areas and about 110 million persons reside in such areas. Fluorosis is a disease involing the whole body, it does harm to almost every system. Fluorosis can hamper child's growth and development, deprive human's labour ability, even make one disabled all the life, which impacts human's health and hinder economical development. So, it is very necessary to explore mechanism of fluorosis and to make prevention measures.Recendy, with the development of free radical medicine, scholars have been averting their studying emphasis to non - bone parament and gradually reach the level of molecular biology and gene. It is very important for human's health to maintain the balance between oxida-tive system and antioxidative system. Data indicate that the balance is broken in a patient with endemic fluorosis, in a result, the body is under the status of oxidative stress, which will change lipid peroxidation. At present, there are some studying data about the relationship between lipid peroxidation and fluorosis, but most of them are animal ex-perimental data. Hence, whether they may be applied to human or not is uncertain. The present study is designed to illustrate the change regularity of lipid peroxidation occurring in the patients with endemic fluorosis and to provide some basic data for the prevention of fluorosis.MethodsEpidemiological investigations and biochemical methods are applied to acquire basic informations about objectives and to monitor the level of lipid peroxidation.Aohanqi county in inner Mongolia is select as survey spot. Fluoride content in drinking water is used as the standard classifying disease areas. Fluoride content in severe fluorosis group ( Liujiedi village) is above 4.0ml/l; and water - improved fluorosis group ( Caosh-eng yingzi village) : under 1.0mg/l after decreasing fluoride content, above 4.0mg/l before the quality of drinking water being improved; medium fluorosis group( Ganggang yingzi village) : between 2. lmg/l and 4.0mg/l; control group(Xindi daguan village) : under 1.0mg/l.Eighteen persons are select as objectives from each studied population who have resided in investigated villages more than fifteen years and matched by age and sex, excluding rheumatoid arthristis and other similar diseases.Venous blood was drew from the objectives who was on an empty stomach in the morning, then reserved these samples in different ways according to respective objectives and brought them back in freeze. (1) GSH content was determined by DTNB method. (2) GSH - PX activity is determined by improved Hafeman method. ( 3 ) LPO content is measured by TEA chromatometry method. (4) SOD activity is de-termined by Nitrite salt methods. (5)Se content is measured by DAN Fluorescence method. (6) Fluoride content in drinking water,,urine and blood is measured by fluoride ion selection electrode method.Ultimate data were analyzed with SPSS 10.0 for windows. Analysis of variance was applied to numerical data and X2 test for categorical variable data, P <0.05 was considered statistically significant.ResultsWater fluoride content is 4 . 68 mg/l in severe fluorosis group ; 2. 22 mg/1 in mild fluorosis group; 0. 95mg/lin water - improved group; 0. 83mg/1 in control fluorosis group. Water fluoride content in respective groups meet the requirement of experiment design. Urine fluoride content varied from each other among severe , medium and control group. The difference is significant( P <0.01) . Urine fluoride content in water - improved group(3.14mg/l) is lower than that of severe group and higher than that of control group( P <0.01).GSH content is respectively 1.41 ±0. 31mg/gHb( severe) Nl. 91 ± 0. 29mg/gHb( water - improved) J. 84 ± 0. 33mg/gHb( mild) ,2.27 ± 0.29 mg/gHb( control) . GSH - PX activity: 58. 68 ± 6.47( severe) , 7. 62 ± 9. 42 ( water - improved ) ,69. 46 ± 9. 96 ( medium ) ,...
|
PDF Full Text Request