Effects Of Curcumin On Blood Lipid, Hepatic Antioxidation Capability And Lipid Metabolism Of Mice Fed With High Fat Diet

The aim of the present research was to investigate the effect of different additivation ratio of curcumin on blood lipid levels,hepatic fuction and the oxidation level of liver in mice fed with high fat diet.the high fat diet was made by highly elevating the ratio of fat and cholesterol on normal mice diet.Excessive intake of fat and energy caused hyperlipidemia and antioxidant capability changes,leading to lipid peroxdation in liver.Forty 8-week-old male C57bl mice were randomly divided into four diet groups with 10 mice in each treatment,mice were housed two to a cage.the diet of groups were include:(1)Nomal diet group,basic nomal mice diet meal of 5% fat by weight(2)High fat diet,a mice diet meal mixed with lard and cholesterol containing 25% fat by weight(3)High fat diet +0.1%cur, high fat diet containing 1g/kg admixture by weight of curcumin(4)High fat diet+l%cur,high fat diet containing 10g/kg admixture of curcuminAll mice remained on their assigned diet until they were killed at the age of 20 Wk, The main results were showed as fellow:1 Long-term excessive intake of high energy food,induced overnutrition station in mice body,significantly increased circulating triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) level in serum of mice. The high-density lipoprotein cholesterol (HDL-C) levels were reduced. Curcumin could reduce serum triglycerides, total cholesterol both with 1g/kg and 10g/kg admixture(P<0.05), 10g/kg admixture of curcumin significantly increased level of high density lipoprotein cholesterol and decrease low-density lipoprotein cholesterol level of serum(P <0.05),tended to maintain the balance of lipid oxidation and fat metabolism of body. Oral administration of both concentrations of curcumin were able to improve the long-term high-fat diet induced hyperlipidemia, 10g/kg admixture of curcumin shows more powful in attenuating hyperlipidemia than 1g/kg admixture of curcumin(P<0.05).2 Mice fed with high fat diet prone to enlargement of liver associate with significantly reducing total antioxidant capacity (T-AOC) (P<0.05) and superoxide dismutase (SOD) activity. lipid peroxidation product malondialdehyde (MDA) was significantly increased in liver.two curcumin treatment both enhance the hepatic SOD activity and T-AOC,at the same time,relieve the MDA content of liver(P<0.05). high fat diet break the redox state of body inducing oxidative stress, Reduced free radical scavenging capacity and increased lipid peroxidation demonstrate some degree of hepatic injury with exccesive fat intake.the decrease of serum ALT and AST also confirmed that two curcumin treatment could relieve liver injury.it appear that lg/kg admixture of curcumin greatly ameliorate hepatic oxidative stress.3 Effect of curcumin on hepatic lipid metabolism.long-term exposed to high fat diet significantly increased hepatic TG and TC content of mice(P<0.05).all of this results show a positive correlation of serum TG and TC level.two curcumin treatments both could relieve the hepatic TG and TC content as compared to high fat diet group. The ameliorative effect shows no significant differece (P>0.05).The hepatic glycogen was significantly decreased in mice fed with high fat diet.1g/kg and 10g/kg admixture of curcumin show significant elevating in hepatic glycogen content.The current study suggest that dietary curcumin can relieve hepatic lipid content and promote the synthesis of glycogen.4 12- weeks high fat diet significantly up-regulated the expression of PPAR-yl(P <0.05), 1g/kg and 10g/kg admixture of curcumin further enhanced the expression of PPAR-yl and This enhancement effect seems to shows a positive correlation with additive amount of curcumin.