| After menopause, the estrogen in women decrease that cause lipid metabolism abnormality and increase cardiovascular disease. Maize purple plant pigment (MPPP) are rich in anthocyanins. MPPP have been reported to have antioxidative and antiperoxiantive activity, to scavenge oxygen radical. But there are no report the effect of MPPP on lipid in lower estrogen levels. Followed the physiological character in postmenopausal women, the ovariectomized rat model was selected to investigate the effect and mechanism of MPPP on lipids, to collect the valuable data of MPPP.Forty Wistar female rats were randomly divided into 5 groups according to body weight, which were sham operation group (SHAM), ovariectomized model group (OVX) and 3 different dose of MPPP+OVX group i.e.2.5%,5.0%,10% MPPP ig lml/100g bw for 14weeks. The body length, body weight, food intakes were recoded. Uterus, liver, perirenal fat and mesenteric fat were dissected and weighted. Blood sample was collected via abdominal aorta from the anaesthetized rat, serum was prepared. Total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) concentrations in serum were determined using automatic biochemical analyzer. The concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in serum and liver were measured, respectively. Data were presented as means and standard deviations, all data were anaylized with one-way ANOVA using SPSS 17.0 statistical software.At study termination, the uterus weights in all OVX groups significantly decreased compared with the sham-operated group (P<0.01), indicating that the ovarian hormone deficiency rat model was well done. In the OVX group, the change rates of food consumption, body weight, Lee's index, perirenal fat coefficient, mesenteric fat coefficient, serum TG, serum HDL-C, MDA concentrations in serum and liver increased as compared with those in the SHAM group (P<0.01 or P<0.05). There was no significant difference in body weight in all MPPP groups and OXV group (P>0.05). Compared with SHAM group, there were higher the change rates of food consumption and Lee's index in all MPPP groups (P<0.001). Perirenal fat coefficient and mesenteric fat coefficient were lower in low MPPP group than that in OVX group (P<0.05). Serum TG, serum HDL-C, MDA concentrations in serum and liver, serum IL-6 increased as compared with those in the SHAM group. Compared with OVX group, serum TC decreased 14.9% and 15.4% in low MPPP group and middle MPPP group, respectively (P<0.05). HDL-C level was lower in low MPPP group and middle MPPP group than that in OVX group (P<0.05). The difference to compare the level of serum TG and LDL-C in low MPPP group,middle MPPP group and OVX group did not have statistics significance (P>0.05). No significant difference was observed in serum TG and LDL-C levels of the five groups (P>0.05). MDA concentrations in serum and liver in low MPPP group and middle MPPP group decreased significantly as compared with that in OVX group (P<0.05), the difference did not have statistics significance as compared with SHAM group(P>0.05).SOD activity in low MPPP group was higher (P<0.01)In summary, ovariectomy in rats induces significant weight gain, fat collect among organs, serum TC increase, antioxidation decrease. MPPP supplement is not able to prevent OVX-induced weight gain in rats. MPPP in optimal dose decreases serum TC level and increases antioxidation in OVX rats. The study results suggest that MPPP may prevent the lipid metabolism abnormality that is due to estrogen deficiency. The effect may correlate with improved anti-oxidation status.
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