The Expressions And Clinical Significances Of Cox-2,p53 In Osteosarcoma

Objective: To observe the expressions of cox-2 and p53 in osteosarcoma and osteochondroma, find the expressional variability of the cox-2 protein and p53 protein in various kinds bone tumor and the correlation between them. To seek a new latent marker of the osteosarcoma, further make use of the latent marker in clinical diagnosis and therapy, to search a effective method in pristine diagnosis and therapy of osteosarcoma, and find a new marker to indicate the prognosis of the osteosarcomar.Methods: Specimens were taken from the department of pathology third hospital of Hebei medical university. 40 specimens from osteosarcoma excision were collected, all of which taken place during 1998-2004.Among them male 24, female 16; The age at time of theraty ranged 8-38 (18.68±6.47); 20 specimens collected during the same period from benign lesion of bone (osteochondroma) operation were taken as the control group. Among them male 13, female 7; The age at time of theraty ranged 9-27 (17.95±4.77).Immunohistochemistry staining (Streptavidin-Peroxidase, S- P method) was performed to detect the expressions of cox-2 protein and p53 protein in 40 cases of osteosarcoma. The criteria of positive expression was that brown granule was found in cytoplasm (cox-2) or in nucleus (p53). The percentage of cox-2 or p53 positive expression in five high power fields were counted randomly in each case. (1) Positive (-): positive oncocytes account <5%; (2) Positive (+):positive oncocytes account 6%-25%; (3) Positive (++):positive oncocytes account 26%-50%; (4) Positive (+++) : positive oncocytes account>50%. The data were analyzed by software SAS v6.12. The difference of cox-2 and p53 expressions between osteosarcoma and osteochondroma was analyzed with Chi-square Test. Spearman method was used to analyze the relationship between cox-2 and p53 expression among osteosarcoma. The standard always adoptedα=0.05.Results: 1 Observed through the optical microscope, the rate of cox-2 expression was 67.5% (27/40) in osteosarcoma, most of them was high. The rate of cox-2 expression was 15%(3/20) in osteochondroma and most of them was low. The positive staining of cox-2 mainly located in cytoplasm. A significant difference was observed between the two group,χ~2 =14.7, P<0.05.2 The positive staining of p53 mainly located in the nucleus of osteosarcoma with the positive rate of 55.0%. There were no expression of p53 in osteochondroma and a significant diference was found between them,χ~2 =17.368, P<0.05.3 There were 18 cases always express positive by cox-2 and p53. And there were 9 cases always express negative by cox-2 and p53. There was a positive correlation between them, rs=0.58,p<0.05.Conclusion: 1 The expressions of cox-2 protein were high in osteosarcoma, but those were low in osteochondroma. There were a noticeable difference between them, it indicate that there was a close correlation between the cox-2 and osteosarcoma. It could be a latent marker in diagnose of osteosarcoma.2 mutant type p53 protein only expressed in osteosarcoma tissues, not in osteochondroma; It indicate that there was a close correlation with the genesis,development,invasion and metastasis of osteosarcoma. It could be a marker in pristine diagnose and prognosis of osteosarcoma.3 The expressions of cox-2 are positively related to that of p53 in osteosarcoma tissues. Cox-2 and p53 may play an important role in the carcinogenesis of osteosarcoma and are correlated with osteosarcoma. The detection of them in clinic might be a guide for the pristine diagnosis and prognosis of osteosarcoma.