Study On Mechanism Of Interferon Treating Pathological Scars

Objective: To research the convey of TGF-β1 and MMPs in human pathological scars (hypertrophic scars and keloids) and the effect of interferon on TGF-β1, MMP-1, PDGF-BB and ppGalNAc - T2 in FB of pathological scars, and the mechanism of interferon on pathological scars. Methods: ten specimen of pathological scars were analysed the expression of TGF-β1 and MMP-1 with the S-P method and were compared with that of healthy skin. The FB of pathological scars were cultured by the method of tissue culture and were randomized in 3 groups: (1)control(0.9%sodium chloride); (2)low concentration (100u/ml INF-α2b); (3)high concentration (10,000 u/ml INF- α2b). Then total groups were analysed the expression of TGF-β1, MMP-1, PDGF-BB, and ppGalNAc - T2 with RT-PCR. Results: (1)The expression of TGF- β1 and MMP-1 were higher in the specimen of pathological scars than in healthy skin by immuno-histochemical method and the result was significantly different (P<0.05) . But the raising scope of TGF-β1, was higher than that of MMP-1. (2)After the effect of 10u/ml INF- α2b and 10,000u/ml INF - α2b on cultured fibroblast of pathological scars, the expression of TGF-β1 , PDGF-BB and ppGalNAc -T2 were lower than that of controlled group and the expression of MMP-1was higher than that of controlled group. The result was significantlydifferent and was related with the concentration of INF- α2b. Conclusion: (1)The expression of TGF-β1 and MMP-1 in specimen of pathological scars were high, but wasn't in normal proportion. Perhaps it is the reason of pathological scars. (2)the reason of the good effect of INF- α 2b on inhibiting fibroblast of pathological scars may be is related with some kinds of cellula factor, such as TGF- β1 PDGF-BB, bFGF, et al.