Effects Of EGF,bFGF,PDGF,TNF-α And NGF On Scars Fibroblasts

[Objective]To explore the mechanism of cytokines for the scars, we study effects of EGF, bFGF, PDGF, TNF-α and NGF on the fibroblasts of scars in biological behavior and pathological change.It can provide the basic of theory in molecular biology and pathobiology.[Materials and MethodsjFibroblasts of scars and normal skins were cultured in vitro. Wounds were created on a Sprague-Dawley (SD) rat model. The results were observed and analysed by light inverted microscope(LM), transmission elextron microscope(TEM), 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5ciphenyltetrazolium bromi -de (MTT) assay, HE staining, Cason's staining and flow cytometry(FCM). It was determined that effects of EGF, bFGF, PDGF, TNF- a and NGF on scars in biological behavior and pathological change.[Results]1. In vitro:using LM, FCM and MTT assay, compared to the control groups, proliferation of fibroblast cells were increased significantly when EGF, bFGF, PDGF, TNF-α and NGF were added to the cultures. 2. In vivo(rat model) rusing pathological staining and TEM, compared to the controls, it could be obtained that the fibroblasts, proliferation was enhanced and collagen synthesis was increased by topically applying EGF, bFGF, PDGF, TNF-# and NGF. [Conclusions]EGF , bFGF , PDGF , TNF- α and NGF can increase fibroblasts proliferation and collagen synthesis, secretion and accumulation. These results demonstrate all of them are beneficial for wound healing at early stage. However, they can lead to pathological scars formation if appllied excessively.