| I PURPOSE1 In order to know virus contamination ( hepatitis B virus as representative virus) of the dental diagnosis and treat instruments and its could bring the potential risks of transmission of viral and bacterial infection during the oral diseases treat process in Tianjin area.2 look for a better disinfection way for the air turbine dental handpieces which could be effective, economic and applicable.II METHOD1 chose one of 3A level hospitals dentistry to carry through sanitation quality investigation of the dental diagnosis and treat instruments. Examined appliance include air turbine dental handpieces, punch-pin, odontoscope, bougie, tweezer, flex-tray that were so common used by dentists and daubed the inside or outside surface of the dental appliance and collected it; Examined targets were HBsAg, HBeAg (detected it with ELISA method ) and HBV-DNA (detected it with molecule spot cross method).2 make good use of the air turbine dental handpieces bracket jack which was all available in every china hospital dentistry and design outside shape and inside structure of the binary disinfectant-box for the air turbine dentalhandpieces. The applicable disinfectant in the cavity of the binary disinfectant-box was high level disinfectant梘lutaraldehyde and according to guideline book of disinfectant evaluation method (ministry of health, P.R.China, 3rd.edition, 1999) germicidal performance and toxicity was tested in the laboratory. Ill RESULT1 HBsAg as target object, the positive rate of air turbine dental handpieces > punch-pin ^ odontoscope x bougie > tweezer, flex-tray was 10.3%> 8.3%> 7.6%, 9.0%, 6.3% > 5.5% respectively. The average positive rate was 7.7%, the air turbine handpieces was the highest 10.3%; HBeAg as target object, the positive rate in turn of the dental instruments said above was \.7%. \.0%. 2.0%. 1.0%> 0.7%, 0 respectively. The average positive rate was 1.1%, air turbine handpieces was the secend highest 1.7%, just next to odontoscope 2.0%; HBV-DNA as target object, the positive rate in turn of the dental instruments was 12.0%, 9.7%. \O.Z%, II^/CK 8.0%> 6.3% respectively. The average positive rate was 9.6%, the air turbine handpieces was the highest 12.0%.2 outside shape and inside structure of the binary disinfectant-boxThe binary disinfectant-box was composed of insert-hand^ airproof-cove^ cavity and the disinfectant stored in cavity. There was another packet which contain another disinfectant or plus-ingredient which located below the airproof-cover. To make use of dental handpieces bracket jack which was all available in every hospital and support and make fast the binary disinfectant-box.3 germicidal performance and toxicity1) The glutaraldehyde content of disinfectant in the cavity of the binary disinfectant-box was about 2.12%, the pH value of glutaraldehyde disinfectant before activation was 3.79, after activation was 6.88;2) The glutaraldehyde content stability test ( accelerating method, 54癈for 14 day) indicated that the glutaraldehyde content of disinfectant come down from 2.12% to 1.94% after stored it in constant temperature box at 54癈 for 14 day, the decreased rate was 8.38% that was less than 10.0% and this disinfectant belong to more stable disinfectant.3) The glutaraldehyde disinfectant in the cavity of the binary disinfectant-box did not cause corrosion of stainless steel > copper and carbon steel and essentially did not cause corrosion of aluminum. The corrosion rate of stainless steel > copper carbon steel and aluminum was 0.000 x 0.0015> 0.0016 and 0.0552 mm/a. We dipped in axletree of dental handpieces for 120 hour with 2.12% glutaraldehyde disinfectant, could not find any metal structure change on the surface of axletree by electronic microscope.4) The antigencity of HBsAg could be destroyed after exposure to disinfectant contains 2.12% glutaraldehyde for 8min. The result of clinical PCR test showed that no HBV snippet found on the either outside surface of the disinfected p...
|
PDF Full Text Request