Study On Disinfectant Of Polyhaxemethylenguanixdine Hydrochloride

Objective: This study covers several aspects concerning disinfection of Polyhaxemethylenguanidine Hydrochloride (PMGH), including disinfection effect, interfering factor, stability, metal causticity as well as toxicology.Methods: Several microorganisms like bacteria and fungi were used as targets for evaluation of disinfection effect of PMGH. The stability of PMGH for the microorganism killing effect, influence factors such as temperature, organic substance, pH value, field experiment for hands disinfection, metal causticity as well as toxicology were systemic studied, respectively.Results: PMGH showed effective in killing all microorganisms tested. Within 1 minute exposure to 0.2% PMGH, 99.97% of streptococci, E.Coli and pseudomonas were terminated, when extends to 10 minutes, all of the microorganisms were destroyed. Within 1 minute exposure to 0.2% PMGH, 99.90% of Candiba albicans was killed. Four hours exposure to 0.6% PMGH resulted in 99.87% Bacillus subtilis spore killed. Field test showed that a minute's exposure to 0.2% PMGH resulted in elimination of 94.27% hands' surface natural bacteria. This disinfectant is stable to temperature and its disinfection effect kept in 54 癈 for 14 days, and 37癈 for 3 months. Organic materials have moderate effect to the disinfection effect, while pH value have a minor effect.Metal causticity study, using stainless steel, carbonized steel, copper and aluminum as targets, showed that PMGH have no effect to stainless steel, carbonized steel and aluminum, have minor effect to copper. Toxicology test showed that half Lethal Dose (LD50) of PMGH for male and female mice was more than 10000mg/kg body weight, which indicates that the disinfectant is actually toxic-free. Skin stimulation test showed that the disinfectant index of stimulation to rabbit skin was zero. Skin allergy experiment using guinea pig as models showed that intensity of allergic reaction was very minor. Micronucleus test showed that this disinfectant did not lead to micronuclogenesis to mouse bone marrowcells.Conclusion: PMGH was demonstrated to kill bacteria in vegetative form and got good disinfection effect, but not to bacterial spore. PMGH was less toxic, minor irritant and no side effect to human skin, good as a disinfectant. This disinfectant is especially suitable for skin and mucous, but sterilization.