Studies On Chemical Constituents And Content Determination Of Ethyl Acetate From

Gentianella acuta, also known as "ku long dan", belongs to the Gentianella Moench. genus in Gentiananceae family. Its whole plant is widely used in Mongolian, Tibetan medicine, with clearing away heat and promoting diuresis, cholagogue effect, strengthening the stomach, callusing and other effects, primarily for the treatment of jaundice, hepatitis, headache, and fever.Gentianella acuta mainly contains xanthones, iridoids, flavonoids, lignans and a small amount of triterpenoids, steroids ingredients. Among them, xanthones and iridoids are the main active ingredients. Modern pharmacological studies have shown that xanthones from Gentianella acuta has a good anti-arrhythmic, improve insulin resistance, protection of ischemic brain damage and antioxidant effects. This paper is divided into three parts: Section 1 ReviewOverview of studies on chemical constituents and pharmacological effects of Gentianella Moench. plants. The plants in this genus mainly contain xanthones, terpenoids, flavonoids, lignin and a few other types of compounds. Among them, xanthones and terpenoids are the main ingredients. The genus has clearing away heat and toxic material, inducing diuresis and reducing edema, and other effects, primarily for the treatment of colds, fever and liver and gallbladder diseases. Modern pharmacological studies have shown that Gentianella turkestanorum has good effect against rheumatoid arthritis.Overview of studies on chemical constituents and pharmacological effects of Gentianella acuta. It mainly contain xanthones, iridoids, flavonoids, lignans and a small amount of triterpenoids, steroids ingredients. Among them, xanthones and iridoids are its main active ingredients. Xanthones from Gentianella acuta have anti-diabetic, anti-arrhythmic, protecting ischemic brain damage, antioxidant, inhibiting acetylcholine activity and other effects; iridoids have hepatoprotective, antioxidant, and other effects. Section 2 Chemical research on the constituents of ethyl acetate extract of Gentianella acutaThe immersion method was used to extract Gentianella acuta, then the ethyl acetate extract crude extract was obtained by solvent extraction. The crude extract was firstly separated by HSCCC, then the fractions were separated and purified by PHPLC and Sephadex LH-20 column chromatography. Finally,14 compounds were obtained, identified as:isorientin (1), norswertianolin (2), swertianolin (3), veratriloside (4),5,8-dihydroxy-1,4-dimethoxy xanthone -3-O-β-D-glucoside (5),1,5-dihydroxy -3,4,8- trimethoxy xanthone (6),1,3,5,8-tetrahydroxy -4- methoxy xanthone (7), demethylbellidifolin (8), swertiabisxanthone-I (9),1,6- dihydroxy -3,4- dimethoxy xanthone (10),1,2,8-trihdroxy-5,6-dimethoxyxanhone (11), corymbiferin (12), bellidifolin (13), oleanolic acid (14). Among them, compounds 5,6,7,9,10,11 were firstly obtained from the plants in this genus. Section 3 Determination of bellidifolin and norswertianolin from Gentianella acuta by HPLCTo establish the quantitative analysis method for bellidifolin and demethylbellidifolin from Gentianella acuta by HPLC. High performance liquid chromatography (HPLC) was performed on a Thermo Syncronis C18 (4.6 mm×250 mm,5 μm). The water with 0.5% phosphoric acid (v/v) was used as an aqueous solvent (A) and CH3CN as an organic solvent (B) with gradient at a flow rate of 1.0mL·min-1. The detection wavelength was 254 nm, and the column temperature was set at 30℃. The method was simple, rapid, accurate and reliable. The contents of bellidifolin and demethylbellidifolin in different parts of Gentianella acuta were determined to provide a scientific basis for quality control and exploitation of Gentianella acuta.