Study On Technique Of Tissue Culture And Micropropagation Of Changjun Chaenomeles

Changjun Chaenomeles is a new good species in Chaenomeles Speciosa, Chaenomeles Lindl, Maloidease, Rosaceae. It is a deciduous leaf bush. In spring, pink flowers, which grow earlier than leaves, are hanging on the branches. In fall, yellow green fruit are hanging from the branches. Between bottle green elliptic shape big leaves and the big stipule off set each other. Its distinguishing feature is especially big, sweet smell easy, thick, fine and smooth pulp, plenty fruit juice. Its processing using rate is up to 90% above. Changjun Chaenomeles is a idea material for processing can, preserved fruit, jelly and drink etc. It is a good species collected view and admire, medicines and edible. The species is introduced from Linyi, Shandong to Yangling, Shaanxi. The number is little. It is not easy taking root to use general vegetative propagation such as process branch, transplant a cutting. Grafting survival rate is low.We have carried out tissue culture of Changjun Chaenomeles in order to supply plentiful seedings with good quality at short period and satisfy the demand in the market. In Vitro, we do the research in the following several periods: initial culture, subculture, rooting and transplant. The technique points and key segment of tissue culture of Changjun Chaenomeles are tentatively explored. The main results are as the following. Initial culture: (1) The explant is sterilized by 84 disinfector in the market (disinfector : water = 1:4) for 15 min, then trandferred into 0.1% HgCI2 for 8 min and washed by sterile water for 4 times.The sterilized effect is good for explants, which are rarely polluted and browned. (2) MS medium is found to be the best medium for the explants of Changjun Chaenomeles according to the experiment of selection of the basal medium. (3) The best concentration of 6-BA, IBA and GA₃ is 0.5 mg/L, 0.1 mg/L and 0.01～0.05 mg/L respectively according to the experiment of selection of hormones. (4) The combination, which is MS+ 6-BA 0.5 mg/L+IBA 0.05 mg/L+ GA₃ is 0.1 mg/L+Agor 4g/L+ Sugar 30 g/L, is excellent for initial culture of Changjun Chaenomeles by L9(3⁴) orthorgnal design. Subculture: (1)The combination, which is MS+ 6-BA 0.5 mg/L+IBA 0.01 mg/L+ GA3 is 0.01 mg/L+Agor 4g/L+ Sugar 30 g/L, is best for subculture of Changjun Chaenomeles by L9(33) orthorgnal design. (2)　The propagation index increases as subculture number is increasing, but propagation index is lightly decreasing after propagation 6 generations.To solve this problem may change the salt concentration of basal medium. (3)It is benefit for obstaining seedings with good quality to add 0.2% activated carbon in the medium. Rooting and tansplant: (1)The three methods for rooting(one step, two steps and without bottle) are experimented. Rooting is obstained by the one step and without bottle.Rooting rate is 45%,75% respectively for Changjun Chaenomeles in rooting one step and without bottle. (2)The rooting seedings obstained by one step are domesticated by adding water before they are transplanted into culture substrate,which is made up vermiculite and river sand(1∶1)。Survial rate is up to 80%。It is necessary to maintain high humidity,substrate ventilation and avoiding pollution。...