Study On Tissue Culture And Cutting Technology Of Chaenomeles Sinensis Koehne

Chaenomeles sinensis is rosaceae papaya woody plants,leaves small trees or shrubs,alias Ming this,because of its dry skin is smooth and does not shrink,also called light skin papaya.Smooth skin papaya is a unique plant in China.It is widely used in food,medicine and ornamental.The ripe fruit is golden in color and fragrant,and is deeply loved by people.In recent years,with the improvement of processing technology,chaenomeles sinensis has become an ideal raw material for canning,candied fruit,juice and jelly.Traditional breeding method is difficult to achieve large-scale production,and the cycle is long,the survival rate is low,by the season and environment impact.In order to get a lot of good seedling in the short term,expand the nursery stock production,this study would take light to make the annual papaya papaya mature branches as experimental material,through the light skin papaya regeneration in vitro culture and cutting technology is studied,and explore the light chaenomeles sinensis the best explant tissue culture and the best way to disinfect,select explant transgenerational original culture,propagation as the best medium cultivation and take root.At the same time,the optimum combination of substrate,hormone and treatment time was selected.The main research contents are as follows:(1)Select the best explant for tissue culture of chaenomeles with smooth skin and the best disinfection methodIn chaenomele tissue culture,the stem segment on the annual twig was used as the explant with the highest success rate and the best growth effect.The best sampling time of chaenomeles sinensis was September in summer,and the contamination rate and mortality of explants after inoculation were significantly lower than those in other spring,autumn and winter.Stem segments were used as explants for tissue culture,and the best disinfection method was as follows: first,the explants were soaked in detergent solution for 1 h,then the surface foam and stains were brushed off,and then the explants were rinsed with running water for 2 hs.Prior to inoculation,alcohol was administered for 20 seconds and 0.1% mercuric chloride for 12 minutes.(2)The screen light chaenomeles sinensis tissue culture the optimal medium for MS basic culture medium,the culture conditions for temperature control in25-30℃,air relative humidity between 60%-70%,every 12 h,light intensity in the 2500-3000 LX.The optimal culture medium for stem segment induction was MS+ 6-BA0.5 mg/L+ NAA 0.1 mg/L+ sucrose 30 g/L+ AGAR 7.0 g/L.The culture time was 35 d,and the average number of induced buds was 3.35.The optimal medium for secondary proliferation was MS+ 6-BA 0.5 mg/L+ NAA 0.1 mg/L+ GA3 0.01 mg/L+sucrose 30 g/L+ AGAR 7.0 g/L.The best medium was MS+ IBA 1.5 mg/L+ sucrose30 g/L+ AGAR 7.0 g/L,followed by dark culture for 7 days,followed by light culture for 28 days.After 14 days of seedling refining,the requirements of transplanting were met.After successful transplanting,the survival rate reached 80%.(3)Establish the chaenomeles sinensis cutting technology system.The process of cutting rooting can be divided into three stages,namely callus formation period,adventitious root induction period and adventitious root elongation period.In late autumn and early winter,the collected chaenomeles sinensis cuttings were buried in winter,and hard cuttings were carried out in the following spring.In march,the rooting rate of hardwood cuttings was the highest(71.2%)in the substrate of peat soil:perlite: vermiculite(3:2:5)after NAA 200 mg/L treatment for 1h.In August,the cuttings were placed in peat soil: perlite: vermiculite(2:1:2)after IBA 400 mg/L treatment for 30 min,and the rooting rate was up to 52.3%.chaenomeles sinensis cuttings rooting,geothermal temperature in more than 15℃,environment temperature is 25-30℃,humidity for more than 90%,the 45 d training,to take root seedling can be obtained.(4)The optimal hormone types,concentrations and matrix conditions for different cuttings were screened out Hardwood cuttage,the optimal hormone species is NAA,the concentration is 200 mg/L,the immersion time of cuttings is 1h,the rooting effect is the best in the substrate of the ratio of peat soil: perlite: vermiculite(3:2:5).The optimal hormone type was IBA,the concentration was 400 mg/L,the soaking time was 30 min,and the rooting effect was the best in the seedbed with peat soil: perlite:vermiculite(2:1:2).