| Phalaenopsis is one of the four world's most commercially valuable ornamental tropical orchids for its characteristics of variety, beautiful color, elegant flower position and a long flowering period. It occupies an extremely important role in flower industry in the world, which has a huge market potential value. The author works in the zhengxin flower technology company in Lianyungang, Jiangsu province, which was founded in 2000, is one of the largest butterfly orchids enterprises for new varieties breeding, propagation, seedling production and export.'Ever GreatDouble Dragon' is one of new varieties of Phalaenopsis amabilis bred in the company in 2009, which has bright velvet red flowers with 12cm diameter and good arrangement in the inflorescence with strong resistance. Especially, more than 95% plants of the new variety have double stems of inflorescence when flowering, which has a good market promising.In this study, the key points of the butterfly orchid stem buds as explants for tissue culture are compared and analyzed in a series of technical studies. The results are showed as following:1. Disinfecting the butterfly orchid stem bud with 2% hydrogen peroxide disinfectant can reduce contamination rates, and improve germination rates. disinfect the stalk for 20min first, and axillary time for 3 min is the best, the contamination rate is 9.43%, the germination rate is 80.53%.The germination rate of the upper bud in butterfly orchid flower stalk stalk was the highest, up to 84.85%; the average length of roots is 2.05cm; the germination rate of stalk buds in middle flower stalk flower was 76.32%, the average length of roots is 2.23cm, and robust than that from other parts. In sum, the production of tissue culture of the middle stem bud from butterfly orchid is the best. The suitable medium for butterfly orchid stem bud induction was 1/3 MS with 3 mg/L 6-BA medium, the induction rate was 86.73%, the budding number was 2.48.Increasing the concentration of 6-BA appropriately is good for Butterfly orchid stem bud differentiation. The best concerntration of 6-BA is 5mg/L, while the germination is 75.64%, the average number of sprouting is 1.76. AD can not benefit the sprouting of butterfly orchid stem bud. It can not be used in the inducing the stalk of butterfly orchid in the practical production.2. The suitable basic medium for proliferation of butterfly orchid stem bud is 1/3MS. Adding appropriate concentration of 6-BA benefits flower stalk buds proliferation. The results showed that 6-BA with a concentration of 5mg/L, proliferation has been up to 1.71 times, which is the best. The buds are robust, and turn dark green and the bud morphology is more normal. Growth potential of each AD treatment is good, indicating that the AD can promote growth of stem bud of butterfly orchid, but it is useless in the proliferation of nodal buds of butterfly orchid. When mixed with 6-BA/AD, proliferation multiples are all higher than used alone, the best ratio of 6-BA/AD is 1:2, the number of new leaves is 2.20, plant height increment is 1.81, the multiplication factor is 1.80 times.The proliferation of multiple shoots of butterfly orchid increases with the coconut milk concentration increased from 0 to 150 ml/L, when the concentration is more than 150 ml/L, there is no more effect. Therefore, the optimal concentration of coconut milk is 150 ml/L.Amount of CH could help the proliferation of flower stalk bud, while excess CH inhibited its growth. The proper CH concentration was 250 mg/L, which produced 82.52% induction rate,1.07 plant height increments,1.25 times proliferation.3. The seedling roots were significantly promoted when medium supplemented with the extract of banana, potato, apple, coconut and activated carbon on. When mixed used, they have the synergistic effect.Gentamicin can more effectively inhibit the endogenous bacterial contamination with the increased concentration, but the rooting rate decreases. The proper concentration is 100 mg/L, the contamination rate is 19.82% and the rooting rate is 91.70% after culturing for 30 days. |
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