Anther Culture Of Jatropha Curcas L. And The Analysis Of PPO And POD In The Callus Of Anther

This paper is divided into two parts; we done the research on the anther culture of Jatropha curcas L., and the effect of hormone and the method of culture on the activity of PPO and POD in the anther callus.In the first part of the paper, we study the inducement of anther callus of Jatropha curcas L., the bud differentiation and the root differentiation by, in order to get the more suitable culture medium. Meanwhile, the suspension culture system is established. The result show that, in the inducement of anther callus, microspores at uninucleate stage were more suitable for culture; the cold pretreatment at 4℃ for 3～5 days is essential to high ratio of inducement; the culture medium that MS+ NAA2.0mg/L +KT0.4mg/L + sucrose 9% is comparatively more suitable for callus inducement, the highest inducement ratio is 40.24%. In the bud differentiation, MS+KT2.0mg/L+NAA0.1mg/L+CH1000mg/L+sucrose 3% is fit. And in the root differentiation, the root can be induced from the callus cultured on the culture medium 1/2MS+IAA0.1mg/L without light. But both the ratio of bud differentiation and root differentiation are low.In the second part of the paper, we research the effect of hormone and the method of culture on the activity of PPO and POD in the anther callus of Jatropha...