Study On Superior Provenance Selection And Tissue Culture Of Jatropha Curcas L

Jatropha curcas L. was used broadly. The seeds contain much more oil. So Jatropha curcas L. was used to produce biologic diesel oil. In this study, provenances of Jatropha curcas L. were collected from Sichuan, Guizhou, Guangxi, Yunnan and Hainan, et al.. The character of seed, endurance of freezing in seedling period, growth of seedlings and index of physiology of different provenances were compared and analyzed. In addition, the technique of tissue culture was studied from sterilizing , choice of explant, application of basal medium, applied concentration of hormones and the main impact rhizogenic factors of rooting culture, transplanting and preventing browning .The main results showed that:1,Superior provenance selection of Jatropha curcas L. There are not much difference in outside character of Jatropha curcas L. provenance,but thousand-seed weight of each provenance has significant difference.(2) There existed differences among different provenances in cold torelence. the strongest cold resistance of the provenances is No.l3(Yunnan,Luchun) and No.14(Guangxi, Guilin),followed by the No.1 (Xilonglin,County),No.2(Guizhou,Wangmo, leyuan),No.3(Guizhou,Wangmo,Sugarcane-township),No.4(Guizhou,Wangmo,Anwu),No.5( Rur al Guizhou Anlong foot), No.10 (Panzhihua, Yanbian), No.12 (Yunnan Yuanyang Ukraine Bay), No.20 ( Hainan), the worst cold resistance is on No.15 (Yunnan Yuanyang Chu Pise) and No.17 (Yunnan Yuanyang Shitouzhai).(3) There are significant differences among various provenance in growth and physiological indicators such as height, diameter, crown growth and Chl content, Car content, Water-retention capacity,Fv/Fm and Yield.3 provenances were tentatively selected,they were NO.2(Guizhou,Wangmo,Leyuan),NO.12(Yunnan,Yuanyang,Ukrainebay),NO.10(Panzhihua,Yanb ian),NO.16(Yunnan,Yuanyang).2,Tissue Culture of Jatropha curcas L.(1) Explant sterilization: The experiment of stem sterilization showed that the stem collected in summer is easier to be sterilized, but the stems should be sterilized immediately after collected. Stems collected from the greenhouse are easier to be sterilized than that collected from field and the younger the stem age, the better the sterilized effects. The best sterilization time is 12min When 0.1% HgCL₂ was used. The results of sterilization on seeds showed that: The seeds stored less than 6 months were easier to be sterilized than those stored 1-2 years and the best sterilization time is 5min when 0.1% HgCL₂ was used.(2)Initial culture: Using stems as the initial explants,the results indicated that the optimal medium for the stems induction was MS+6-BA0.3mg/L, the induction rate reaching to 63.3%.When we use the embryos as explants,MS with 6-BA0.8mg/L and 25℃was the best combination for embryos of induce buds, the induction rate was 61.7%.MS+NAA0.2mg/L was found to be suitable for embryos of induce callus.(3)Culture of multiplication: l/2MS+6-BA0.5mg/L was the best propagation mediun, in which the multiplication rate can be achieved more than four times.When the callus from embryo put in MS+6-BA0.3mg/L+KT0.05mg/L,the number of buds we get was 4.07 on average.(4)Rooting culture: The optimal medium for plant rooting was MS+NAA1.2mg/L,the root growth rate can be reached to 56.67%.(5)Transplanting:The optimal transplanting medium was the margarite and yellow soil which are mixed with 1:1.Experiment showed that the transplant survival rate can be reached to 58%.(6)Control of browning:The ascorbic acid showed the best effects in preventing browning.The rate of browning reduce to 6.67% when the concentration was 50mg/L.