Effect Of Sunshine Intensity And Fertilizer Application On Growth,Nutrients Absorption And Content Of Isoflavone In Red Clover

Agavision (Trifolium pretense L.), a variety of red clover, was used to study the effect of sunshine intensity and fertilizer application on growth and isoflavone in red clover with two different experiments i.e. the sunshine intensity experiment and the fertilizer application experiment. In the sunshine intensity experiment, four different sun-shading levels were set likely no sun-shading as CK, 15% of sun-shiding as S1, 25% of sun-shading as S2 and 45% of sun-shading as S3. Four different fertilizer treatments in the fertilizer application experiment likely no fertilizer application as CK, N (symbol of nitrogen) applied singly as A, N+P (symbol of nitrogen + phosphorus) applied as B, and N+P+K (symbol of nitrogen + phosphorus + kalium, applied as C. To study the effect of the two factors, plant height, branch, rhizome width, element contents, isoflavone contents etc. were investigated. The results ate as follows:1 Modirate sun-shading may help to heighten plant and help branching, and the effect of the excess sun-shading treatments was less for heightening and turned to restrain for branching. Sun-shading also restrained, in some measure, the development of rhizome. And the effect more distinct, the shading rate bigger. The correlation analyse indicated there are distinct negative correlation between rhizome diameter and shading rate(r=-0.950*). The single plant yield reduced with the rate of the shading bigger, and that are distinct differences between treatments in the prophase and the anaphase. The distinct positive correlation between the single plant yield and the rhizome diameter(r=0.997*), and high positive correlation between the single plant yield and the height of plant and the amount of branch, the distinct positive correlation and most distinct correlation between the yield and the stem mass (r=0.950*) and leaf mass(r=0.992**).2 With the devolepment process, the N content in stem changed with a "V" shape, and increased as a approximate line in leaf; the P content in stem reduced, and changed as a parabola in leaf; the K content in stem and leaf increased firstly, men reduced. Sun-shading course the content of N, P, K. increased, the gradation by the N content was as S1> S2 > S3 - CK, and as S3 >S2 >S1 >CK by the P and K content The high positive correlation between the N content and the single plant yield, and low correlation between the yield and P, K content.3 With the devolepment process, the content of isoflavone increased firstly, men reduced, sun-shading enhenced the content of isoflavone in stem, and restrain the content in leaf where the restrain effect increased with the rate of shading bigger. The yield of isoflavone reduced with the shading rate bigger, and the highest value emerge in 10s of Nov. there's distinct positive correlation between the yield of isoflavone and rhizome diameter.4 Fertilizer enhenced the devolepment of red clover, for example, enhenced the plant height, esp. the N+P treatment did best; enhenced branching, the N+P+Kdid best; enhenced the rhizome diameter, the N+P and N+P+K treatments best. Fertilizer enhenced distinctly the accumulations of the dry matter in stem and leaf, and the treatments lined by the effect as N+P+K>N+P≈ N>>CK. while the differences between the treatments aren't distinct.5 Fertilizer had effect on the accumulation of N, P and K in different organs. N fertilized only enhanced the absorbability of N in stem and leaf, and the N content of the others didn't change by distinct rugulation. The contents of P reduce as the time passing, the N applied cause the P content reduced in stem and increased in leaf, and the N+P didn't increase the content of P in stem, but restrain some extent. And K could reduce the restrain effect. The K content increased firstly, then reduced with the devolepment process, in different fertilizer treatment, the K content changed by indistinct regulation.6 Fertilizer caused the content of isoflavone reduced, as the order no fertilizer >N+P+K>N>N+P, mainly caused by the content of the leaf. Because fertilizer enhenced the aboveground plant yield, the aboveground yield of isoflavone increased.