Studies On Microstructure And Ultrastructure Of Acrida Chinesis Oogenesis

Using microscopy and electron microscopy oogenesis in Acrida cinerea Thunberg has been investigated. Oogenesis was described briefly and the ways of the formation of yolk granules were explored.At first,eight stages in the oogenesis of the Acrida chinensis were distinguished and described respectively, basing on the changes of the oocyte's size, chromosomal changes in the nucleus of the oocyte, vitellogenesis and the size of the follicle cells.Secondly, the trends of DNA and RNA in the oocytes and follicle cells were displayed by histochemical method during oogenesis, which indicated that RNA were synthesized at stage of the vitellogenic oocytes. But the quantity of DNA wasn't change. RNA showed a positive Pyronin reaction in follicle cells after vitellogenesis beginning.lt indicated that follicle cells perhaps takcd part in the synthesis of yolk precursor. In the oogenesis of the Acrida chinensis, patency played a very important role in the process of fertilization. The nutritive material was transported directly from the hemolymph passed through the follicle cells and then into the oocytes.Ultrastructural features of oogenesis of the Acrida chinensis were observed under TEM. Genesis of yolk granule and transformation of cell organelle in the oocytes of the Acrida chinensis were investigated. It has been found that five cell parts, i.e. mitochondria, SER vesicles, Golgi vesicles, multivesicular bodies and micropinocytotic vesicles, may be involved in the formation of yolk granules. Yolk synthesis appeared to occure by both autosynthetic and heterosynthetic process. The follicle cells taked part in the synthesis of yolk precursor, and the yolk precursor were transported into oocyte by pinocytosis and fusion. After follicle cells secreting egg shells, oogenesis completed.