Clinical And Mechanism Study Of Treating Osteoarthritis Of The Knee With "Modified Of Aconitum Decoction"

Purpose:In this paper,the clinical observation and experimental study were conducted based on the exact therapeutic effect of "modified of aconitum decoction" prescription for Knee Osteoarthritis(KOA),and the mechanism was analyzed by bioinformatics.From the perspective of biology and modern pharmacology,it provides the exact basis for the treatment of KOA by "modified of aconitum decoction".Method:?.Clinical Observation on Treating Knee Osteoarthritis with "modified of aconitum decoction" PrescriptionSixty patients with KOA from the Second Hospital of Traditional Chinese Medicine in Jiangsu Province were recruited.The TCM constitution scores of KOA patients were assessed using the TCM quality table,and the data of the body mass table were statistically analyzed to determine the constitutional tendency of the KOA patients.The clinical application of square treatment of KOA provides theoretical basis for traditional Chinese medicine in terms of constitution.We analyzed 72 cases of wind-cold-type KOA patients treated with compound prescriptions of "modified of aconitum decoction" in hospital or outpatient department of our hospital.VAS scores before and after treatment,knee joint HSS scores,efficacy evaluation scores,and safety were observed,and their comprehensive evaluation Efficacy.?.Pharmacological study of "modified of aconitum decoction" recipe for the treatment of KOAThe study on the compound of "modified of aconitum decoction" was conducted in cell and animal experiments to verify the efficacy of KOA treatment and explore its mechanism of action.Specific studies of cell experiments include:1)cytotoxicity studies;2)inflammatory factor inhibition experiments;3)NO(nitric oxide free radical)inhibition experiments.The specific research contents of animal experiments include:1)Establishment and grouping of KOA rat model;2)Model validation;3)Therapeutic effect of Wutou decoction on KOA;Study of histopathological examination of rat knee cartilage;4)Safety Evaluation.?.Analysis of bioinformatics analysis and mechanism of compound prescription of"modified of aconitum decoction"The bioinformatics research method was used to analyze the potential targets of the compound components of "modified of aconitum decoction",including gene ontology(GO)and OMIM/TTD disease concentration analysis.Combined with the existing literature and research results,the mechanism of the compound treatment KOA was presumed.Result1.Among the KOA patients,the physique of Yang deficiency was the most,followed by Yin deficiency,Qi deficiency,and blood stasis related constitution.2.After treatment with "modified of aconitum decoction" orally,the VAS score of the experimental group was significantly lower than that of the control group(p<0.05).It was found that the external application and physiotherapy of Chinese herbal medicine Wutou Decoction combined with plaster can effectively relieve The pain of patients with knee osteoarthritis of wind and cold type improves knee function and improves their quality of life without adverse reactions.3.On the two levels of animals and cells,traditional Chinese medicine decoction can effectively reduce the expression of IL-1?,TNF-?,IL-6 and other inflammatory factors,and also inhibit the expression of NO and PGE2 in cells.Less than the positive control resveratrol,significant anti-inflammatory activity.4.Analysis revealed that multiple protein targets of NF-kB signaling pathway are related to Aconitum decoction.conclusionThe constitution investigation of TCM found that in the KOA patients,the physique of Yang deficiency is the most,and the correlation with the cold and wet type in the clinical syndrome differentiation is larger;the treatment of wind-cold and wet-type KOA is performed through the differentiation of "Wuxuetang plus flavor" recipe.Clinical studies have found that its efficacy is significant and there are no adverse reactions,suggesting that this side can effectively treat KOA;through pharmacological studies,it is found that "modified of aconitum decoction" can effectively inhibit inflammatory factors(IL-1?,IL-6,TNF-? The production of,the low toxicity to mice and cells;through bioinformatics analysis,combined with the above studies and relevant literature,speculated that "modified of aconitum decoction" can inhibit the activation of related proteins by inhibiting the activation of NF-?B signaling pathway,Reduce the expression of inflammatory factors.This study demonstrated that "modified of aconitum decoction" is an effective drug for the treatment of wind-cold-type KOA.