| ObjectivePelvic inflammatory disease,CPID is a common gynecological disease.The main systems of CPID include abdominal pain,backache,abnormal leucorrhea,which can eventually lead to infertility,ectopic fetation and other complications.All of those impact on women's health seriously and increase the family and social economic burden.Western medicine treatments include antibiotics,physical therapy are unfavorable for chronic inflammatory.Traditional Chinese medical therapy which include taking in traditional Chinese medicine,Chinese patent drug and traditional Chinese medicine enemata,achieve positive effect.In clinically,damp heat and blood stasis syndrome is the most common type in CPID.Kun Fu Kang capsule follow the principle of promoting blood circulation for removing blood stasis,and clearing away heat and promoting diuresis has obvious effect in long-term clinical application.This study was supported by the Shanxi Huikang pharmaceutical Limited company.The study try to clarify the influence on red cell immunology by ways of traditional Chinese medicine working on the CPID,and to elucidate the pathogenesis of this disease.MethodWe collected 70 cases which are diagnosed CPID(damp heat and blood stasis syndrome)in the First Affiliated Hospital of Guangzhou University of TCM from March 2016 to October 2016.The 70 patients were classified into the two groups randomly:observation group(Kun Fu Kang capsule),control group(Hua Hong capsule),with each group having 35 patients.6 cases were lost,and 64 cases were completed finally.The observation group was prescribed with Kun Fu Kang capsule 3 tablets at a time and three times one day.The control group was prescribed with Kun Fu Kang capsule 4 tablets at a time and three times one day,20 days for a course,treatment of 2 courses,and withdrawal in menstrual.To observe the changes of clinical symptoms,signs,peripheral blood cells,thromboxane B2(TXB2),6-Ketoprostaglandin Fla(6-K-PGla),erythrocyte C3b receptor rosette rate(E-C3bRR),erythrocyte immune complexes rosette rate(E-ICR),rosette forming enhancing rate of erythrocyte C3b receptor(RFER),rosette forming inhibitory rate of erythrocyte C3b receptor(RFIR),Interleukine-8(IL-8),Interleukine-10(IL-10),Duffy antigen/receptor for chemokine(DARC),superoxide dismutase(SOD),malondialdehyde(MDA),erythrocyte CD59 after the treatment.Result1.Clinical observation:After treatment,the scores of the observation group were significantly lower than the control group(P<0.05).The scores of the two groups were significantly lower than those before treatment(P<0.01).Compared with the control group,the sign and synthetical curative effect of the observation group was significantly better than the control group(P<0.01),but the curative effect of TCM syndrome was not significant difference(P>0.05).2.Peripheral blood cell test:The observation group compared with the control group,before and after treatment,the levels of peripheral blood cell count were not statistically significant(P>0.05).Compared with the observation group before and after treatment the levels of lymphocyte count was increased,neutrophil count and neutrophil/lymphocyte ratio decreased(P<0.05).Compared with the control group before and after treatment,the levels of the neutrophil/NLR decreased after treatment(P<0.05).3.Plasma TXB2,6-P-KGla detection:After treatment,the TXB2 and 6-P-KGla of observation group was significantly lower than those of the control group(P<0.05).Compared with the observation group before and after treatment the levels of 6-P-KGla was increased,TXB2 decreased(P<0.01).Compared with the control group before and after treatment,the levels of TXB2 was decreased(P<0.01),6-P-KGla was not statistically significant(P>0.05).4.E-C3bRR?E-ICR?REER?RFIR detection:After treatment,the E-C3bRR of the observation group was significantly higher and the E-ICR was significantly lower than those of the control group(P<0.05).Compared with the observation group before and after treatment the levels of E-C3bRR was increased,E-ICR decreased,REER increased(P<0.01).Compared with the control group before and after treatment,the levels of E-C3bRR was increased,E-ICR decreased(P<0.05),REER was not statistically significant(P>0.05).The observation group compared with the control group,the two groups before and after the comparison of RFIR were not statistically significant(P>0.05).There is a negative correlation between E-C3bRR and NLR and TXB2,and the absolute value of correlation coefficient is close to zero,indicating that the degree of close relationship is not close.5.The expression of CD59 in erythrocyte:After treatment,the erythrocyte CD59 of the observation group was significantly higher than that of the control group(P<0.05).Compared with the observationl group before and after treatment,the levels of erythrocyte CD59 was increased(P<0.01).Compared with the control group before and after treatment,the levels of erythrocyte CD59 was not statistically significant(P>0.05).6.Plasma IL-8,IL10,IL-8/IL10,erythrocyte DARC detection:After treatment,the IL-8/IL10 of the observation group was significantly lower and the erythrocyte DARC was significantly higher than those of the control group(P<0.05).Compared with the observationl group before and after treatment,the levels of IL-8 was decreased,IL10 and erythrocyte DARC increased(P<0.05).Compared with the control group before and after treatment,the levels of IL-8 was decreased(P<0.05).Erythrocyte DARC had a negative correlation with IL-8,IL-8/IL10(P<0.05),and a positive correlation with IL-10(P<0.05).There was a positive correlation between IL-8 and IL10(P<0.01).However,their absolute value of correlation coefficient was close to 0,which showed that the correlation degree was not close.7.Erythrocyte SOD,plasma MDA detection:The levels of SOD and serum MDA in erythrocytes were significantly higher than those in the control group(P<0.01),and there was no significant difference between the two groups(P>0.05).Compared with the observationl group before and after treatment,the levels of erythrocyte SOD was increased(P<0.01).Compared with the control group before and after treatment,the levels of SOD was increased(P<0.05).There was no significant difference in MDA between the two groups(P>0.05).SOD and MDA had a negative correlation(P<0.01),but the absolute value of the correlation coefficient is close to 0.5,indicating that the degree of closeness is general.8.Correlation analysis between damp heat and blood stasis type CPID synthetical score and experimental index:There was correlation between NLR,TXB2,E-C3bRR,E-ICR,SOD,CD59,IL-8,IL-8/IL-10,erythrocyte DARC and damp heat and blood stasis type CPID synthetical score.Where the absolute value of E-C3bRR is the closest to 1,indicating that the correlation is greatest.Conclusion1.In clinical trials,Kun Fu Kang capsule to improve the patient's lower abdominal pain,backache,vaginal discharge,abdominal pain,menstrual disorders(menstrual flow or prolonged menstrual extension)and other clinical symptoms(total effective rate of 100%),ease the pelvic local signs(Total effective rate of 100%),for the damp heat and blood stasis syndrome type CPID have a good therapeutic effect(total effective rate of 100%),and the latter two Kun Fu Kang capsule is better than the control drug bonus tablets.2.Can reduce the peripheral blood NLR,improve the hemadynamics by increasing TXB2,reducing 6-P-KGla and improve the content and activity of CR1,SOD,CD59 and DARC in erythrocytes and decrease the proinflammatory cytokines IL-8.Multi-channel treatment of CPID had a good result.And in improving the immune function of erythrocytes,Kun Fu Kang capsule is better than the effect of bonus tablets.3.NLR,TXB2,E-ICR,IL-8,IL-8/IL-10 and damp heat and blood stasis type CPID synthetical score was positively correlated.6-P-KG,E-C3bRR,REER,SOD,CD59,DARC and damp heat and blood stasis type CPID score was negatively correlated.Among them,E-C3bRR has the greatest degree of correlation,suggesting that red blood cell CR1 immune function in the CPID mechanism research has important theoretical and application value. |
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