Study Of Risk Factors And Polymorphisms Of Apolipoprotein E And Apolipoprotein AV In Han And Uygur Populations With Gallstone

Objective:(1)To explore the prevalence rate of gallstone and the risk factors of gallstone in Han and Uygur population in One Center;(2) to investigate the relationship between ApoE gene polymorphism and gallstones;(3) to investigate the relationship between ApoAV gene tagging SNPs polymorphism and gallstones. Methods:(1)9455cases in one hospital Health Care Center were used to analyze the prevalence rates of gallstones in different nationalities, moreover, to explore the risk factors of gallstones in different ethnic populations (Han and Uygur) and in different gender and different ages by the non-conditional Logistic regression; and also to explore the different risk factors in different groups;(2) A case-control study including80Uygur gallstones and56controls as well as64Han gallstones and60controls was designed to observe the relationship between the polymorphisms of ApoE and gallstones by the restriction fragment length polymorphism;(3) A case-control study including80Uygur gallstones and56controls as well as64Han gallstones and60controls was designed to observe the relationship between the polymorphisms of ApoAV and gallstones by the restriction fragment length polymorphism. Results:(1)The prevalence rate of gallstone is different in different ethnics、genders and ages. The prevalence rate of gallstone in Uygur populations is significantly higher than the Han nationality (22.87%vs11.64%, P<0.05), and so does in different ages(24.30%vs7.82%, P<0.05), but there is no difference in different genders(12.88%vs13.51%, P>0.05). The risk factors of gallstone is partly different in different ages and genders. Interestingly, the ethnic is the common risk factor. So we furtherly compared the risk factors and general data of gallstone in Han and Uygur, we found that there are different risk factors and different general characteristics in different ethnics. In Han groups ages、TG、BMI and HDL is risk factors and ages、TC、female and fatty Live is risk factors in Uygur groups. Aged、female、fatty Live、more BMI、higher TC and higher LDL is common in Uygur groups;(2) The distribution of ApoE gene types is not found difference between Han and Uygur groups, allele ε3is the common gene type in general population. The frequency of ε4and ε2is higher in Uygur than in Han, but there is no statistical difference. In total groups, it is the fact that the frequency of s4is higher as well as the frequency of s2is lower. The distribution of allelic of ApoE is same in different ethnics. Further analysis found that e2is the protectable gene type for gallstone when compared with the non-ε2gene types (OR=0.51vs OR=0.53, P<0.05),ε4maybe is the risk gene types when compared with the non-84gene types(OR=1.22vs OR=1.26, P>0.05). We concluded that the risk factors of gene is maybe the combianed changed of the epsilon2decereased and epsilon4incereased, comparing with the interpretion that one epsilon decreased or increased.(3)The tag SNP of ApoAV genotype distribution is not found difference in gallstone groups and controls both in rs2266788and rs2075294. rs2266788is also no difference in Han and Uygur populations but rs2075294T allele is significantly lower in Uygur than that in Han. Further analysis found that rs2266788and rs2075294are in linkage disequilibrium haplotype. The statistical analysis is also no association between ApoAV haplotype and gallstone. OR of tag SNPs in gallstone is greater than one, but no statistical significance. There is also no statistical significance in serum lipid between different gene types. Conclusions:(1) The prevalence of gallstone is higher in Uygur population and aged groups. The risk factors are different in different ages and genders. Ethnic is an important risk factors in gallstone. The general characterics and risk factors are different in different ethnics;(2) The distribution of ApoE gene types is not found difference between Han and Uygur groups. Epsilon2(an protectable gene type)decerased combained epsilon4(maybe an risk gene type) increased which maybe the risk factors for gallstone could be found in gallstone groups in total groups;(3)ApoAV gene types are not associated with GST and serum lipids, but the T alleles of rs2075294is lower in Uygur compared with Han.